| RRC ID |
74805
|
| 著者 |
Satou C, Neve RL, Oyibo HK, Zmarz P, Huang KH, Arn Bouldoires E, Mori T, Higashijima SI, Keller GB, Friedrich RW.
|
| タイトル |
A viral toolbox for conditional and transneuronal gene expression in zebrafish.
|
| ジャーナル |
Elife
|
| Abstract |
The zebrafish is an important model in systems neuroscience but viral tools to dissect the structure and function of neuronal circuitry are not established. We developed methods for efficient gene transfer and retrograde tracing in adult and larval zebrafish by herpes simplex viruses (HSV1). HSV1 was combined with the Gal4/UAS system to target cell types with high spatial, temporal, and molecular specificity. We also established methods for efficient transneuronal tracing by modified rabies viruses in zebrafish. We demonstrate that HSV1 and rabies viruses can be used to visualize and manipulate genetically or anatomically identified neurons within and across different brain areas of adult and larval zebrafish. An expandable library of viruses is provided to express fluorescent proteins, calcium indicators, optogenetic probes, toxins and other molecular tools. This toolbox creates new opportunities to interrogate neuronal circuits in zebrafish through combinations of genetic and viral approaches.
|
| 巻・号 |
11
|
| 公開日 |
2022-7-22
|
| DOI |
10.7554/eLife.77153
|
| PII |
77153
|
| PMID |
35866706
|
| PMC |
PMC9307271
|
| MeSH |
Animals
Gene Expression
Neurons / physiology
Optogenetics / methods
Rabies virus* / genetics
Zebrafish* / genetics
|
| IF |
7.08
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(vglut1-hs:GFP)
Tg(vglut1-hs:Gal4)
Tg(gad1b:GFP)
Tg(vglut2a:loxP-DsRed-loxP-GFP)
Tg(UAS:tdTomato-CAAX)rw0315
Tg(vglut1:GFP)
Tg(vglut1:Gal4) |
