RRC ID 74851
著者 Yao D, Guo J, Qin T, Chen H, Jin S.
タイトル Effect of Alleviating Fibrosis with EGCG-Modified Bone Graft in Murine Model Depended on Less Accumulation of Inflammatory Macrophage.
ジャーナル Biomed Res Int
Abstract In response to current trends in the modification of guided bone regeneration (GBR) materials, we aimed to build upon our previous studies on epigallocatechin-3-gallate (EGCG) by immersing a commonly used bone graft primarily composed of hydroxyapatite (HA) in EGCG solution, expecting to obtain superior bone material integration after implantation. Bone grafts are commonly used for bone repair, in which the bone extracellular matrix is stimulated to promote osteogenesis. However, due to its profibrosis effect, this osteoconductive material commonly exhibits implant failure. In addition to providing a basic release profile of EGCG-modified bone graft (E-HA) to clarify the relationship between this material and the environment, we have examined the integration effect via subcutaneous implantation experiments. In this manner, we have assessed the aggregation of proinflammatory macrophages, the formation of fibrous capsules, and an enhanced cell viability observed in cultured RAW 264.7 cells. Among these results, we focus on proinflammatory macrophages due to their close relationship with fibrosis, which is the most important process in the immune response. Immunofluorescent staining results showed that E-HA substantially compromised the formation of fibrous capsules in hematoxylin-eosin-stained sections, which exhibited less proinflammatory macrophage recruitment; meanwhile, the cell viability was improved. This work lays the foundation for future studies on GBR.
巻・号 2023
ページ 9466110
公開日 2023-1-1
DOI 10.1155/2023/9466110
PMID 36820222
PMC PMC9938781
MeSH Animals Bone Regeneration / physiology Capsules / pharmacology Catechin* / pharmacology Disease Models, Animal Durapatite / pharmacology Macrophages Mice Osteogenesis*
IF 2.276
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)