| RRC ID |
74878
|
| Author |
Nakagawa Y, Hishida T, Sumaru K, Morishita K, Kirito K, Yokojima S, Sakamoto Y, Nakamura S, Uchida K.
|
| Title |
Phototunable Cell Killing by Photochromic Diarylethene of Thiazoyl and Thienyl Derivatives.
|
| Journal |
J Med Chem
|
| Abstract |
We report a unique phototunable cell killing technique using diarylethene molecules as photo-isomerizing-molecular switches. These molecules were delivered to DNA in the cell nucleus due to closed-form generated by UV light, and then blue light triggered cell killing. A UV light irradiation switches the open form, having no DNA intercalation activity, to the closed form to induce intercalation in DNA. This isomer, thus prepared ready for the action, exerts photocytotoxicity upon the subsequent blue light irradiation. Molecular biological analysis clarifies that photocytotoxicity is due to DNA double-strand breaks. Since cell death is observed only when irradiated with light where both the open- and closed-ring isomers have absorption, the possible mechanism of cell death is assumed to be due to the repeated photocyclization and photocycloreversion reactions of the diarylethene molecules, which induce irreparable damage to DNA. This unique photo-controllable action in a cell system can provide the basis of a novel scheme of phototherapy.
|
| Volume |
66(8)
|
| Pages |
5937-5949
|
| Published |
2023-4-27
|
| DOI |
10.1021/acs.jmedchem.3c00164
|
| PMID |
37128763
|
| MeSH |
Cell Death
Ethylenes*
Isomerism
Light*
Molecular Structure
|
| IF |
6.205
|
| Resource |
| Human and Animal Cells |
HeLa(RCB0007) |
