RRC ID |
74892
|
著者 |
Asano-Inami E, Yokoi A, Sugiyama M, Hyodo T, Hamaguchi T, Kajiyama H.
|
タイトル |
The association of UBAP2L and G3BP1 mediated by small nucleolar RNA is essential for stress granule formation.
|
ジャーナル |
Commun Biol
|
Abstract |
Stress granules (SGs) are dynamic, non-membranous structures composed of non-translating mRNAs and various proteins and play critical roles in cell survival under stressed conditions. Extensive proteomics analyses have been performed to identify proteins in SGs; however, the molecular functions of these components in SG formation remain unclear. In this report, we show that ubiquitin-associated protein 2-like (UBAP2L) is a crucial component of SGs. UBAP2L localized to SGs in response to various stresses, and its depletion significantly suppressed SG organization. Proteomics and RNA sequencing analyses found that UBAP2L formed a protein-RNA complex with Ras-GTP-activating protein SH3 domain binding protein 1 (G3BP1) and small nucleolar RNAs (snoRNAs). In vitro binding analysis demonstrated that snoRNAs were required for UBAP2L association with G3BP1. In addition, decreased expression of snoRNAs reduced the interaction between UBAP2L and G3BP1 and suppressed SG formation. Our results reveal a critical role of SG component, the UBAP2L/snoRNA/G3BP1 protein-RNA complex, and provide new insights into the regulation of SG assembly.
|
巻・号 |
6(1)
|
ページ |
415
|
公開日 |
2023-4-14
|
DOI |
10.1038/s42003-023-04754-w
|
PII |
10.1038/s42003-023-04754-w
|
PMID |
37059803
|
PMC |
PMC10104854
|
MeSH |
Carrier Proteins* / genetics
Carrier Proteins* / metabolism
DNA Helicases* / genetics
DNA Helicases* / metabolism
Poly-ADP-Ribose Binding Proteins / genetics
Poly-ADP-Ribose Binding Proteins / metabolism
RNA Helicases / genetics
RNA Helicases / metabolism
RNA Recognition Motif Proteins / genetics
RNA Recognition Motif Proteins / metabolism
RNA, Small Nucleolar / genetics
Stress Granules
|
IF |
4.165
|
リソース情報 |
ヒト・動物細胞 |
HeLa(RCB3680)
293T(RCB2202) |