RRC ID 74892
著者 Asano-Inami E, Yokoi A, Sugiyama M, Hyodo T, Hamaguchi T, Kajiyama H.
タイトル The association of UBAP2L and G3BP1 mediated by small nucleolar RNA is essential for stress granule formation.
ジャーナル Commun Biol
Abstract Stress granules (SGs) are dynamic, non-membranous structures composed of non-translating mRNAs and various proteins and play critical roles in cell survival under stressed conditions. Extensive proteomics analyses have been performed to identify proteins in SGs; however, the molecular functions of these components in SG formation remain unclear. In this report, we show that ubiquitin-associated protein 2-like (UBAP2L) is a crucial component of SGs. UBAP2L localized to SGs in response to various stresses, and its depletion significantly suppressed SG organization. Proteomics and RNA sequencing analyses found that UBAP2L formed a protein-RNA complex with Ras-GTP-activating protein SH3 domain binding protein 1 (G3BP1) and small nucleolar RNAs (snoRNAs). In vitro binding analysis demonstrated that snoRNAs were required for UBAP2L association with G3BP1. In addition, decreased expression of snoRNAs reduced the interaction between UBAP2L and G3BP1 and suppressed SG formation. Our results reveal a critical role of SG component, the UBAP2L/snoRNA/G3BP1 protein-RNA complex, and provide new insights into the regulation of SG assembly.
巻・号 6(1)
ページ 415
公開日 2023-4-14
DOI 10.1038/s42003-023-04754-w
PII 10.1038/s42003-023-04754-w
PMID 37059803
PMC PMC10104854
MeSH Carrier Proteins* / genetics Carrier Proteins* / metabolism DNA Helicases* / genetics DNA Helicases* / metabolism Poly-ADP-Ribose Binding Proteins / genetics Poly-ADP-Ribose Binding Proteins / metabolism RNA Helicases / genetics RNA Helicases / metabolism RNA Recognition Motif Proteins / genetics RNA Recognition Motif Proteins / metabolism RNA, Small Nucleolar / genetics Stress Granules
IF 4.165
リソース情報
ヒト・動物細胞 HeLa(RCB3680) 293T(RCB2202)