論文 - 詳細
| RRC ID | 74900 |
|---|---|
| 著者 | Kawasaki S, Ono H, Hirosawa M, Kuwabara T, Sumi S, Lee S, Woltjen K, Saito H. |
| タイトル | Programmable mammalian translational modulators by CRISPR-associated proteins. |
| ジャーナル | Nat Commun |
| Abstract |
Translational modulation based on RNA-binding proteins can be used to construct artificial gene circuits, but RNA-binding proteins capable of regulating translation efficiently and orthogonally remain scarce. Here we report CARTRIDGE (Cas-Responsive Translational Regulation Integratable into Diverse Gene control) to repurpose Cas proteins as translational modulators in mammalian cells. We demonstrate that a set of Cas proteins efficiently and orthogonally repress or activate the translation of designed mRNAs that contain a Cas-binding RNA motif in the 5'-UTR. By linking multiple Cas-mediated translational modulators, we designed and built artificial circuits like logic gates, cascades, and half-subtractor circuits. Moreover, we show that various CRISPR-related technologies like anti-CRISPR and split-Cas9 platforms could be similarly repurposed to control translation. Coupling Cas-mediated translational and transcriptional regulation enhanced the complexity of synthetic circuits built by only introducing a few additional elements. Collectively, CARTRIDGE has enormous potential as a versatile molecular toolkit for mammalian synthetic biology. |
| 巻・号 | 14(1) |
| ページ | 2243 |
| 公開日 | 2023-4-19 |
| DOI | 10.1038/s41467-023-37540-7 |
| PII | 10.1038/s41467-023-37540-7 |
| PMID | 37076490 |
| PMC | PMC10115826 |
| MeSH | Animals CRISPR-Associated Proteins* / genetics CRISPR-Cas Systems* / genetics Gene Expression Regulation Gene Regulatory Networks Mammals / genetics RNA, Messenger |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 40 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB3677) 1383D6(HPS1006) |