| RRC ID |
74902
|
| 著者 |
Shoenhard H, Jain RA, Granato M.
|
| タイトル |
The calcium-sensing receptor (CaSR) regulates zebrafish sensorimotor decision making via a genetically defined cluster of hindbrain neurons.
|
| ジャーナル |
Cell Rep
|
| Abstract |
Decision making is a fundamental nervous system function that ranges widely in complexity and speed of execution. We previously established larval zebrafish as a model for sensorimotor decision making and identified the G-protein-coupled calcium-sensing receptor (CaSR) to be critical for this process. Here, we report that CaSR functions in neurons to dynamically regulate the bias between two behavioral outcomes: escapes and reorientations. By employing a computational guided transgenic strategy, we identify a genetically defined neuronal cluster in the hindbrain as a key candidate site for CaSR function. Finally, we demonstrate that transgenic CaSR expression targeting this cluster consisting of a few hundred neurons shifts behavioral bias in wild-type animals and restores decision making deficits in CaSR mutants. Combined, our data provide a rare example of a G-protein-coupled receptor that biases vertebrate sensorimotor decision making via a defined neuronal cluster.
|
| 巻・号 |
41(10)
|
| ページ |
111790
|
| 公開日 |
2022-12-6
|
| DOI |
10.1016/j.celrep.2022.111790
|
| PII |
S2211-1247(22)01678-3
|
| PMID |
36476852
|
| PMC |
PMC9813870
|
| MeSH |
Animals
Receptors, Calcium-Sensing* / genetics
Zebrafish* / genetics
|
| IF |
8.109
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(glyt2:Gal4) |
