論文 - 詳細
| RRC ID | 74929 |
|---|---|
| 著者 | Kim YS, Sohn SH, Min TJ. |
| タイトル | Protective Effect of Ulinastatin on Cognitive Function After Hypoxia. |
| ジャーナル | Neuromolecular Med |
| Abstract |
Ulinastatin (UTI) has neuroprotective properties. Neurologic insults, including hypoxia and use of anesthetic agents, cause postoperative cognitive dysfunction and alter gamma-aminobutyric acid (GABA) function. This study aimed to assess whether UTI could preserve learning and memory using a zebrafish hypoxic behavior model and biomarkers. Zebrafish (6-8 months of age and 2.5-3.5 cm long) were divided into eight groups as follows: phosphate-buffered saline (PBS) control, hypoxia + PBS, UTI (10,000, 50,000, and 100,000 units/kg), and hypoxia with UTI (10,000, 50,000, and 100,000 units/kg) groups. The endpoints of the T-maze experiment included total time, distance moved, and frequency in target or opposite compartment. We also measured the degree of brain infarction using 2,3,5‑triphenyltetrazolium chloride staining, assessed SA-β-galactosidase activity, and examined GABAA receptor expression using real-time polymerase chain reaction. In a dose-dependent manner, UTI affected learning and memory in zebrafish. Despite hypoxia, 100,000 units/kg of UTI preserved preference (time and distance) for the target compartment. More than 50,000 units/kg of UTI also showed reduced hypoxia-induced brain infarction, decreased SA-β-galactosidase levels, and upregulated GABAA receptors. This study demonstrated that the location of the GABAA receptor is affected by hypoxia or UTI. |
| 巻・号 | 25(1) |
| ページ | 136-143 |
| 公開日 | 2023-3-1 |
| DOI | 10.1007/s12017-022-08721-2 |
| PII | 10.1007/s12017-022-08721-2 |
| PMID | 35917079 |
| MeSH | Animals Brain Infarction Cognition Hypoxia / drug therapy Receptors, GABA-A* Zebrafish* gamma-Aminobutyric Acid |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(gad1b:GFP) |