RRC ID 74943
Author Shimura T, Suehiro T, Suzuki H, Okada K, Araki K, Asao T, Kuwano H.
Title The soluble form of human leukocyte antigen class I antigen causes apoptosis on hepatocellular carcinoma cell lines.
Journal Oncol Rep
Abstract A soluble form of human leukocyte antigen class I antigen (sHLA-I) has been reported to cause apoptosis on cytotoxic T cells and inhibit killer activity of natural killer cells via killer-cell inhibitory receptors. However, its effect on cancer cells has not yet been elucidated. We examined the direct effect of sHLA-I on human liver cancer cell lines, HepG2, HLE and HLF. The effects of sHLA-I on cell growth, DNA synthesis, and apoptosis induction were evaluated. To elucidate the mechanisms, cDNA expression arrays were also examined. sHLA-I caused cell growth inhibition, resulting in apoptosis on human hepatocellular carcinoma, dose-dependently. In this process, caspase-3 was activated. sHLA-I also inhibited in vivo growth of hepatocellular carcinoma in severe combined immunodeficient mice. sHLA-I caused apoptosis on human hepatocellular carcinoma.
Volume 16(6)
Pages 1375-80
Published 2006-12-1
PMID 17089064
MeSH Alternative Splicing Animals Antineoplastic Agents / isolation & purification Antineoplastic Agents / pharmacology* Apoptosis / drug effects* Blotting, Western Carcinoma, Hepatocellular / pathology* Cell Line, Tumor Cell Proliferation / drug effects Electrophoresis, Polyacrylamide Gel Histocompatibility Antigens Class I / isolation & purification Histocompatibility Antigens Class I / pharmacology* Humans Immunoenzyme Techniques Liver Neoplasms / pathology* Mice Mice, SCID Neoplasm Transplantation Oligonucleotide Array Sequence Analysis Protein Isoforms / isolation & purification Protein Isoforms / pharmacology
IF 3.417
Resource
Human and Animal Cells Hep G2