Reference - Detail
| RRC ID | 74986 |
|---|---|
| Author | Takahashi S, Ferdousi F, Yamamoto S, Hirano A, Nukaga S, Nozaki H, Isoda H. |
| Title | Botryococcus terribilis Ethanol Extract Exerts Anti-inflammatory Effects on Murine RAW264 Cells. |
| Journal | Int J Mol Sci |
| Abstract |
The present study aimed to evaluate the effects of Botryococcus terribilis ethanol extract (BTEE) on lipopolysaccharide (LPS)-induced inflammation in RAW264 cells. BTEE significantly attenuated LPS-induced nitric oxide production and inflammatory cytokines release, including Ccl2, Cox2, and Il6. On the other hand, several anti-inflammatory mediators, such as Pgc1β and Socs1, were increased in BTEE-treated cells. Further, we performed an untargeted whole-genome microarray analysis to explore the anti-inflammatory molecular mechanism of BTEE. Enrichment analysis showed BTEE significantly downregulated 'response to stimulus', 'locomotion', and 'immune system response' and upregulated 'cell cycle' gene ontologies in both 6- and 17-h post-LPS stimulation conditions. Pathway analysis revealed BTEE could downregulate the expressions of chemokines of the CC and CXC subfamily, and cytokines of the TNF family, TGFβ family, IL1-like, and class I helical. PPI analysis showed AXL receptor tyrosine kinase (Axl), a receptor tyrosine kinase from the TAM family, and its upstream transcription factors were downregulated in both conditions. Node neighborhood analysis showed several Axl coexpressed genes were also downregulated. Further, kinase enrichment and chemical perturbation analyses supported Axl inhibition in BTEE-treated conditions. Altogether, these findings suggest anti-inflammatory effects of BTEE that are mediated via the suppression of pro-inflammatory cytokines and predict its potential as an Axl inhibitor. |
| Volume | 24(7) |
| Published | 2023-4-3 |
| DOI | 10.3390/ijms24076666 |
| PII | ijms24076666 |
| PMID | 37047640 |
| PMC | PMC10095501 |
| MeSH | Animals Anti-Inflammatory Agents / metabolism Anti-Inflammatory Agents / pharmacology Cytokines / metabolism Ethanol* / pharmacology Lipopolysaccharides / pharmacology Macrophages* / metabolism Mice NF-kappa B / metabolism Nitric Oxide / metabolism Nitric Oxide Synthase Type II / metabolism RAW 264.7 Cells |
| IF | 4.556 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |