論文 - 詳細
| RRC ID | 74987 |
|---|---|
| 著者 | Porat J, El Baidouri M, Grigull J, Deragon JM, Bayfield MA. |
| タイトル | The methyl phosphate capping enzyme Bmc1/Bin3 is a stable component of the fission yeast telomerase holoenzyme. |
| ジャーナル | Nat Commun |
| Abstract |
The telomerase holoenzyme is critical for maintaining eukaryotic genome integrity. In addition to a reverse transcriptase and an RNA template, telomerase contains additional proteins that protect the telomerase RNA and promote holoenzyme assembly. Here we report that the methyl phosphate capping enzyme (MePCE) Bmc1/Bin3 is a stable component of the S. pombe telomerase holoenzyme. Bmc1 associates with the telomerase holoenzyme and U6 snRNA through an interaction with the recently described LARP7 family member Pof8, and we demonstrate that these two factors are evolutionarily linked in fungi. Our data suggest that the association of Bmc1 with telomerase is independent of its methyltransferase activity, but rather that Bmc1 functions in telomerase holoenzyme assembly by promoting TER1 accumulation and Pof8 recruitment to TER1. Taken together, this work yields new insight into the composition, assembly, and regulation of the telomerase holoenzyme in fission yeast as well as the breadth of its evolutionary conservation. |
| 巻・号 | 13(1) |
| ページ | 1277 |
| 公開日 | 2022-3-11 |
| DOI | 10.1038/s41467-022-28985-3 |
| PII | 10.1038/s41467-022-28985-3 |
| PMID | 35277511 |
| PMC | PMC8917221 |
| MeSH | Holoenzymes / genetics Holoenzymes / metabolism Phosphates / metabolism RNA / metabolism Schizosaccharomyces* / metabolism Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism Telomerase* / genetics Telomerase* / metabolism Telomere / genetics Telomere / metabolism |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY6843 |