論文 - 詳細
| RRC ID | 75002 |
|---|---|
| 著者 | Nakajima H, Ishikawa H, Yamamoto T, Chiba A, Fukui H, Sako K, Fukumoto M, Mattonet K, Kwon HB, Hui SP, Dobreva GD, Kikuchi K, Helker CSM, Stainier DYR, Mochizuki N. |
| タイトル | Endoderm-derived islet1-expressing cells differentiate into endothelial cells to function as the vascular HSPC niche in zebrafish. |
| ジャーナル | Dev Cell |
| Abstract |
Endothelial cells (ECs) line blood vessels and serve as a niche for hematopoietic stem and progenitor cells (HSPCs). Recent data point to tissue-specific EC specialization as well as heterogeneity; however, it remains unclear how ECs acquire these properties. Here, by combining live-imaging-based lineage-tracing and single-cell transcriptomics in zebrafish embryos, we identify an unexpected origin for part of the vascular HSPC niche. We find that islet1 (isl1)-expressing cells are the progenitors of the venous ECs that constitute the majority of the HSPC niche. These isl1-expressing cells surprisingly originate from the endoderm and differentiate into ECs in a process dependent on Bmp-Smad signaling and subsequently requiring npas4l (cloche) function. Single-cell RNA sequencing analyses show that isl1-derived ECs express a set of genes that reflect their distinct origin. This study demonstrates that endothelial specialization in the HSPC niche is determined at least in part by the origin of the ECs. |
| 巻・号 | 58(3) |
| ページ | 224-238.e7 |
| 公開日 | 2023-2-6 |
| DOI | 10.1016/j.devcel.2022.12.013 |
| PII | S1534-5807(22)00895-4 |
| PMID | 36693371 |
| MeSH | Animals Endoderm Endothelial Cells* Endothelium Hematopoietic Stem Cells / physiology Zebrafish* |
| IF | 10.092 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(UAS:GFP) Tg(UAS:RFP) |