| RRC ID |
75053
|
| 著者 |
Ana Sousa-Ortega, Javier Vázquez-Marín, Estefanía Sanabria-Reinoso, Jorge Corbacho, Rocío Polvillo, Alejandro Campoy-López, Lorena Buono, Felix Loosli, María Almuedo-Castillo, Juan R. Martínez-Morales
|
| タイトル |
A Yap-dependent mechanoregulatory program sustains cell migration for embryo axis assembly
|
| ジャーナル |
Nature Communications
|
| Abstract |
The assembly of the embryo’s primary axis is a fundamental landmark for the establishment of the vertebrate body plan. Although the morphogenetic movements directing cell convergence towards the midline have been described extensively, little is known on how gastrulating cells interpret mechanical cues. Yap proteins are well-known transcriptional mechanotransducers, yet their role in gastrulation remains elusive. Here we show that the double knockout of yap and its paralog yap1b in medaka results in an axis assembly failure, due to reduced displacement and migratory persistence in mutant cells. Accordingly, we identified genes involved in cytoskeletal organization and cell-ECM adhesion as potentially direct Yap targets. Dynamic analysis of live sensors and downstream targets reveal that Yap is acting in migratory cells, promoting cortical actin and focal adhesions recruitment. Our results indicate that Yap coordinates a mechanoregulatory program to sustain intracellular tension and maintain the directed cell migration for embryo axis development.
|
| 巻・号 |
14
|
| 公開日 |
2023-5-16
|
| DOI |
10.1038/s41467-023-38482-w
|
| PMID |
37193708
|
| PMC |
PMC10188487
|
| MeSH |
Adaptor Proteins, Signal Transducing* / genetics
Adaptor Proteins, Signal Transducing* / metabolism
Cell Movement / genetics
Focal Adhesions / genetics
Focal Adhesions / metabolism
Transcription Factors* / genetics
Transcription Factors* / metabolism
YAP-Signaling Proteins
|
| IF |
12.121
|
| リソース情報 |
| メダカ |
NBRP medaka website database |
