論文 - 詳細
| RRC ID | 75054 |
|---|---|
| 著者 | Nakayama M. |
| タイトル | VCre/VloxP and SCre/SloxP as Reliable Site-Specific Recombination Systems for Genome Engineering. |
| ジャーナル | Methods Mol Biol |
| Abstract |
The Cre/loxP system is a versatile and powerful tool that has been used to develop many kinds of genetically modified mice, such as conditional knockout mice and mutant protein-expressing mice through the excision of a STOP cassette. However, while numerous in vivo and in vitro applications of the Cre/loxP system have been reported, it remains difficult to target at one time more than one set of recognition sites in an identical single cell in mice using the Cre/loxP system. To overcome this barrier, we developed two novel site-specific recombination systems called VCre/VloxP and SCre/SloxP. These systems allow multiple independent site-specific recombination, for example, multiple targeted deletions in the same cell at different times. In this chapter, I describe the features of VCre/VloxP and SCre/SloxP, practical protocols and tips on how to use them in genomic engineering applications, potential problems in their use, and how problems can be identified and solved. |
| 巻・号 | 2637 |
| ページ | 161-180 |
| 公開日 | 2023-1-1 |
| DOI | 10.1007/978-1-0716-3016-7_13 |
| PMID | 36773146 |
| MeSH | Animals Genome* Genomics Integrases* / genetics Mice Mice, Knockout Recombination, Genetic |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | B6N Mouse BAC clone (RDB07573) |