RRC ID 75054
Author Nakayama M.
Title VCre/VloxP and SCre/SloxP as Reliable Site-Specific Recombination Systems for Genome Engineering.
Journal Methods Mol Biol
Abstract The Cre/loxP system is a versatile and powerful tool that has been used to develop many kinds of genetically modified mice, such as conditional knockout mice and mutant protein-expressing mice through the excision of a STOP cassette. However, while numerous in vivo and in vitro applications of the Cre/loxP system have been reported, it remains difficult to target at one time more than one set of recognition sites in an identical single cell in mice using the Cre/loxP system. To overcome this barrier, we developed two novel site-specific recombination systems called VCre/VloxP and SCre/SloxP. These systems allow multiple independent site-specific recombination, for example, multiple targeted deletions in the same cell at different times. In this chapter, I describe the features of VCre/VloxP and SCre/SloxP, practical protocols and tips on how to use them in genomic engineering applications, potential problems in their use, and how problems can be identified and solved.
Volume 2637
Pages 161-180
Published 2023-1-1
DOI 10.1007/978-1-0716-3016-7_13
PMID 36773146
MeSH Animals Genome* Genomics Integrases* / genetics Mice Mice, Knockout Recombination, Genetic
DNA material B6N Mouse BAC clone (RDB07573)