| RRC ID |
75124
|
| Author |
Ortiz de la Rosa JM, Nordmann P, Zong Z, Poirel L.
|
| Title |
Aliidiomarina shirensis as Possible Source of the Integron- and Plasmid-Mediated Fosfomycin Resistance Gene fosC2.
|
| Journal |
Antimicrob Agents Chemother
|
| Abstract |
In-silico analysis and cloning experiments identified a fosC2-like fosfomycin resistance gene in the chromosome of Aliidiomarina shirensis, with our data suggesting that this bacterium might be added to the list of species identified as reservoirs of fos-like genes that were subsequently acquired by other Gram-negative species. Indeed, the fosC2 gene was identified as acquired in Providencia huaxinensis and Aeromonas hydrophila isolates, with this gene being located in class 1 integron structures in the latter cases. Biochemical characterization and site-directed mutagenesis showed a higher catalytic efficiency for the intrinsic FosC2AS (from A. shirensis) than for the acquired FosC2 (from P. huaxinensis) enzyme due to a single substitution in the amino acid sequence (Gly43Glu). Notably, this study constitutes the first identification of the likely natural reservoir of a complete gene cassette (including its attC site).
|
| Volume |
66(3)
|
| Pages |
e0222721
|
| Published |
2022-3-15
|
| DOI |
10.1128/aac.02227-21
|
| PMID |
35041510
|
| PMC |
PMC8923222
|
| MeSH |
Anti-Bacterial Agents / pharmacology
Fosfomycin* / pharmacology
Gammaproteobacteria
Integrons / genetics
Microbial Sensitivity Tests
Plasmids / genetics
Polymerase Chain Reaction
|
| Resource |
| General Microbes |
JCM 17761 |
