| RRC ID |
75125
|
| Author |
Ekimova GA, Fedorov DN, Doronina NV, Khmelenina VN, Mustakhimov II.
|
| Title |
AcdR protein is an activator of transcription of 1-aminocyclopropane-1-carboxylate deaminase in Methylobacterium radiotolerans JCM 2831.
|
| Journal |
Antonie Van Leeuwenhoek
|
| Abstract |
It has been previously shown that a number of plant associated methylotrophic bacteria contain an enzyme aminocyclopropane carboxylate (ACC) deaminase (AcdS) hydrolyzing ACC, the immediate precursor of ethylene in plants. The genome of the epiphytic methylotroph Methylobacterium radiotolerans JCM2831 contains an open reading frame encoding a protein homologous to transcriptional regulatory protein AcdR of the Lrp (leucine-responsive regulatory protein) family. The acdR gene of M. radiotolerans was heterologously expressed in Escherichia coli and purified. The results of gel retardation experiments have shown that AcdR specifically binds the DNA fragment containing the promoter-operator region of the acdS gene. ACC decreased electrophoretic mobility of the AcdR-DNA complex whereas leucine had no effect on the complex mobility. The mutant strains of M. radiotolerans obtained by insertion of a tetracycline cassette in the acdS or acdR gene lost the ACC-deaminase activity but the strains with complementation of the mutation recovered this function. The acdS- mutant but not acdR- strain expressed the xylE reporter gene under the control of acdS promoter region thus resulting in a catechol 2,3-dioxygenase activity. This suggested that AcdR in vivo functions as activator of transcription of the acdS gene. The results obtained in this study showed that in phytosymbiotic methylotroph Methylobacterium radiotolerans AcdR mediates activation of the acdS gene transcription in the presence of an inducer ACC or 2-aminoisobutyrate and the excess of the regulatory protein assists in transcription initiation even in the absence of the inducer. The model of regulation of acdS transcription in M. radiotolerans was proposed.
|
| Volume |
115(9)
|
| Pages |
1165-1176
|
| Published |
2022-9-1
|
| DOI |
10.1007/s10482-022-01764-2
|
| PII |
10.1007/s10482-022-01764-2
|
| PMID |
35867173
|
| MeSH |
Carbon-Carbon Lyases* / genetics
Carbon-Carbon Lyases* / metabolism
Escherichia coli / genetics
Escherichia coli / metabolism
Methylobacterium* / genetics
Methylobacterium* / metabolism
Promoter Regions, Genetic
Transcription, Genetic
|
| Resource |
| General Microbes |
JCM 2831 |
