| RRC ID |
75191
|
| Author |
Dengler L, Meier J, Grünberger F, Bellack A, Rachel R, Grohmann D, Huber H.
|
| Title |
Methanofollis propanolicus sp. nov., a novel archaeal isolate from a Costa Rican oil well that uses propanol for methane production.
|
| Journal |
Arch Microbiol
|
| Abstract |
A novel methanogenic strain, CaP3V-MF-L2AT, was isolated from an exploratory oil well from Cahuita National Park, Costa Rica. The cells were irregular cocci, 0.8-1.8 μm in diameter, stained Gram-negative and were motile. The strain utilized H2/CO2, formate and the primary and secondary alcohols 1-propanol and 2-propanol for methanogenesis, but not acetate, methanol, ethanol, 1-butanol or 2-butanol. Acetate was required as carbon source. The novel isolate grew at 25-40 °C, pH 6.0-7.5 and 0-2.5% (w/v) NaCl. 16S rRNA gene sequence analysis revealed that the strain is affiliated to the genus Methanofollis. It shows 98.8% sequence similarity to its closest relative Methanofollis ethanolicus. The G + C content is 60.1 mol%. Based on the data presented here type strain CaP3V-MF-L2AT (= DSM 113321T = JCM 39176T) represents a novel species, Methanofollis propanolicus sp. nov.
|
| Volume |
204(9)
|
| Pages |
554
|
| Published |
2022-8-13
|
| DOI |
10.1007/s00203-022-03152-w
|
| PII |
10.1007/s00203-022-03152-w
|
| PMID |
35962867
|
| PMC |
PMC9375759
|
| MeSH |
1-Propanol
Archaea* / genetics
Costa Rica
DNA, Archaeal / genetics
Methane
Methanomicrobiaceae* / genetics
Oil and Gas Fields
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| Resource |
| General Microbes |
JCM 39176 |
