論文 - 詳細
| RRC ID | 75222 |
|---|---|
| 著者 | Mizutani T, Hara R, Takeuchi M, Yamagishi K, Hirao Y, Mori K, Hibi M, Ueda M, Ogawa J. |
| タイトル | l-Tryptophan-starved cultivation enhances S-allyl-l-cysteine synthesis in various food-related microorganisms. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
S-Allyl-l-cysteine (SAC) has received much interest due to its beneficial effects on human health. To satisfy the increasing demand for SAC, this study aims to develop a valuable culturing method for microbial screening synthesizing SAC from readily available materials. Although tryptophan synthase is a promising enzyme for SAC synthesis, its expression in microorganisms is strictly regulated by environmental l-tryptophan. Thus, we constructed a semisynthetic medium lacking l-tryptophan using casamino acids. This medium successfully enhanced the SAC-synthesizing activity of Lactococcus lactis ssp. cremoris NBRC 100676. In addition, microorganisms with high SAC-synthesizing activity were screened by the same medium. Food-related Klebsiella pneumoniae K-15 and Pantoea agglomerans P-3 were found to have a significantly increased SAC-synthesizing activity. The SAC-producing process established in this study is shorter in duration than the conventional garlic aging method. Furthermore, this study proposes a promising alternative strategy for producing food-grade SAC by microorganisms. |
| 巻・号 | 86(6) |
| ページ | 792-799 |
| 公開日 | 2022-5-24 |
| DOI | 10.1093/bbb/zbac044 |
| PII | 6564596 |
| PMID | 35388878 |
| MeSH | Antioxidants / metabolism Cysteine* / chemistry Garlic* / chemistry Humans Tryptophan / metabolism |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 18462 JCM 18460 JCM 1113 JCM 18461 |