| RRC ID |
75222
|
| 著者 |
Mizutani T, Hara R, Takeuchi M, Yamagishi K, Hirao Y, Mori K, Hibi M, Ueda M, Ogawa J.
|
| タイトル |
l-Tryptophan-starved cultivation enhances S-allyl-l-cysteine synthesis in various food-related microorganisms.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
S-Allyl-l-cysteine (SAC) has received much interest due to its beneficial effects on human health. To satisfy the increasing demand for SAC, this study aims to develop a valuable culturing method for microbial screening synthesizing SAC from readily available materials. Although tryptophan synthase is a promising enzyme for SAC synthesis, its expression in microorganisms is strictly regulated by environmental l-tryptophan. Thus, we constructed a semisynthetic medium lacking l-tryptophan using casamino acids. This medium successfully enhanced the SAC-synthesizing activity of Lactococcus lactis ssp. cremoris NBRC 100676. In addition, microorganisms with high SAC-synthesizing activity were screened by the same medium. Food-related Klebsiella pneumoniae K-15 and Pantoea agglomerans P-3 were found to have a significantly increased SAC-synthesizing activity. The SAC-producing process established in this study is shorter in duration than the conventional garlic aging method. Furthermore, this study proposes a promising alternative strategy for producing food-grade SAC by microorganisms.
|
| 巻・号 |
86(6)
|
| ページ |
792-799
|
| 公開日 |
2022-5-24
|
| DOI |
10.1093/bbb/zbac044
|
| PII |
6564596
|
| PMID |
35388878
|
| MeSH |
Antioxidants / metabolism
Cysteine* / chemistry
Garlic* / chemistry
Humans
Tryptophan / metabolism
|
| リソース情報 |
| 一般微生物 |
JCM 18462
JCM 18460
JCM 1113
JCM 18461 |
