論文 - 詳細
| RRC ID | 75225 |
|---|---|
| 著者 | Palberg D, Kisiała A, Jorge GL, Emery RJN. |
| タイトル | A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones. |
| ジャーナル | BMC Microbiol |
| Abstract |
BACKGROUND:Symbiotic Methylobacterium strains comprise a significant part of plant microbiomes. Their presence enhances plant productivity and stress resistance, prompting classification of these strains as plant growth-promoting bacteria (PGPB). Methylobacteria can synthesize unusually high levels of plant hormones, called cytokinins (CKs), including the most active form, trans-Zeatin (tZ). RESULTS:This study provides a comprehensive inventory of 46 representatives of Methylobacterium genus with respect to phytohormone production in vitro, including 16 CK forms, abscisic acid (ABA) and indole-3-acetic acid (IAA). High performance-liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) analyses revealed varying abilities of Methylobacterium strains to secrete phytohormones that ranged from 5.09 to 191.47 pmol mL-1 for total CKs, and 0.46 to 82.16 pmol mL-1 for tZ. Results indicate that reduced methanol availability, the sole carbon source for bacteria in the medium, stimulates CK secretion by Methylobacterium. Additionally, select strains were able to transform L-tryptophan into IAA while no ABA production was detected. CONCLUSIONS:To better understand features of CKs in plants, this study uncovers CK profiles of Methylobacterium that are instrumental in microbe selection for effective biofertilizer formulations. |
| 巻・号 | 22(1) |
| ページ | 49 |
| 公開日 | 2022-2-8 |
| DOI | 10.1186/s12866-022-02454-9 |
| PII | 10.1186/s12866-022-02454-9 |
| PMID | 35135483 |
| PMC | PMC8822675 |
| MeSH | Chromatography, High Pressure Liquid / methods Cytokinins / analysis* Cytokinins / metabolism* Methylobacterium / chemistry* Methylobacterium / classification Methylobacterium / genetics* Methylobacterium / metabolism Tandem Mass Spectrometry / methods |
| リソース情報 | |
| 一般微生物 | JCM 16406 JCM 2805 JCM 2806 JCM 2803 JCM 20693 JCM 16407 JCM 14648 JCM 10893 JCM 14673 JCM 14674 |