論文 - 詳細
| RRC ID | 75230 |
|---|---|
| 著者 | Afrizal A, Jennings SAV, Hitch TCA, Riedel T, Basic M, Panyot A, Treichel N, Hager FT, Wong EO, Wolter B, Viehof A, von Strempel A, Eberl C, Buhl EM, Abt B, Bleich A, Tolba R, Blank LM, Navarre WW, Kiessling F, Horz HP, Torow N, Cerovic V, Stecher B, Strowig T, Overmann J, Clavel T. |
| タイトル | Enhanced cultured diversity of the mouse gut microbiota enables custom-made synthetic communities. |
| ジャーナル | Cell Host Microbe |
| Abstract |
Microbiome research needs comprehensive repositories of cultured bacteria from the intestine of mammalian hosts. We expanded the mouse intestinal bacterial collection (www.dsmz.de/miBC) to 212 strains, all publicly available and taxonomically described. This includes strain-level diversity, small-sized bacteria, and previously undescribed taxa (one family, 10 genera, and 39 species). This collection enabled metagenome-educated prediction of synthetic communities (SYNs) that capture key functional differences between microbiomes, notably identifying communities associated with either resistance or susceptibility to DSS-induced colitis. Additionally, nine species were used to amend the Oligo-Mouse Microbiota (OMM)12 model, yielding the OMM19.1 model. The added strains compensated for phenotype differences between OMM12 and specific pathogen-free mice, including body composition and immune cells in the intestine and associated lymphoid tissues. Ready-to-use OMM stocks are available for future studies. In conclusion, this work improves our knowledge of gut microbiota diversity in mice and enables functional studies via the modular use of isolates. |
| 巻・号 | 30(11) |
| ページ | 1630-1645.e25 |
| 公開日 | 2022-11-9 |
| DOI | 10.1016/j.chom.2022.09.011 |
| PII | S1931-3128(22)00467-X |
| PMID | 36208631 |
| MeSH | Animals Bacteria Disease Models, Animal Gastrointestinal Microbiome* / genetics Intestines Mammals / genetics Metagenome Mice Microbiota* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 128 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 一般微生物 | JCM 39204JCM 39205JCM 39206 |