| RRC ID |
75260
|
| 著者 |
Liu Y, Zha H, Yu S, Zhong J, Liu X, Yang H, Zhu Q.
|
| タイトル |
Molecular characterization and antibacterial activities of a goose-type lysozyme gene from roughskin sculpin (Trachidermus fasciatus).
|
| ジャーナル |
Fish Shellfish Immunol
|
| Abstract |
Lysozymes, acting as antimicrobial molecules, play a vital role in the host's innate immune response to pathogen infections. In the present study, a g-type lysozyme gene termed Tf-LyzG from roughskin sculpin, Trachidermus fasciatus was firstly reported. The deduced amino acid sequence of Tf-LyzG contained 188 residues and possessed conserved catalytic residues (Glu71, Asp84, and Asp95). Gene expression analysis revealed that Tf-LyzG was widely distributed in the tested eleven tissues with the highest expression in the gill and could be significantly induced post lipopolysaccharide (LPS) challenge. The lysozyme activity of the purified recombinant protein (rTf-LyzG) was found to be most active at pH 5.5 and 37 °C. rTf-LyzG exhibited a wide spectrum of potent bacteriolytic activity against four Gram-positive bacteria and six Gram-negative bacteria. It also displayed a high affinity to polysaccharides on bacteria surfaces including LPS, lipoteichoic acid (LTA), and peptidoglycan (PGN). rTf-LyzG was capable of binding and agglutinating all nine bacteria. Flow cytometry assay further revealed that rTf-LyzG could disrupt the membrane of Micrococcus lysodeikticus which is confirmed by scanning electron microscope (SEM) analysis that reveals blebs around the bacterial cell membrane. In summary, these data indicate that Tf-LyzG is of great importance in the fish immune response against pathogens invasion.
|
| 巻・号 |
127
|
| ページ |
1079-1087
|
| 公開日 |
2022-8-1
|
| DOI |
10.1016/j.fsi.2022.07.053
|
| PII |
S1050-4648(22)00442-9
|
| PMID |
35870746
|
| MeSH |
Animals
Anti-Bacterial Agents
Base Sequence
Geese / genetics
Geese / metabolism
Immunity, Innate / genetics
Lipopolysaccharides / pharmacology
Muramidase*
Perciformes*
Phylogeny
|
| リソース情報 |
| 一般微生物 |
JCM 1464 |
