| RRC ID |
75287
|
| 著者 |
Noor Mohammadi T, Shen C, Li Y, Zayda MG, Sato J, Masuda Y, Honjoh KI, Miyamoto T.
|
| タイトル |
Characterization of Clostridium perfringens bacteriophages and their application in chicken meat and milk.
|
| ジャーナル |
Int J Food Microbiol
|
| Abstract |
Clostridium perfringens is one of the most important foodborne pathogens in developed countries. It causes severe food poisoning outbreaks worldwide, along with mortality and economic losses. Recently, bacteriophages have been investigated as an alternative tool to control pathogenic bacteria in the food industry. In this study, 19 Clostridium perfringens and 6 Clostridium perfringens bacteriophages were isolated from chicken meat. According to host range and stability tests, bacteriophage CPQ1 showed high thermostability and the broadest host range. The electron micrograph image of this bacteriophage suggested that it belongs to the Picovirinae subfamily of the Podoviridae family. Nucleotide sequence analysis of the genomic DNA indicated the absence of any antibiotic resistance, toxin, or virulence genes. In broth, CPQ1 showed strong lytic activity with a low MOI of 1, decreasing the OD600 of Clostridium perfringens cell suspension from 0.2 to 0.02 at 37 °C in 2 h. In pasteurized milk and chicken meat, CPQ1 with an MOI of 10 also caused a significant decrease in viable counts of Clostridium perfringens compared to the bacteriophageless control at both 24 °C and 37 °C. This is the first report on the application of bacteriophage to control Clostridium perfringens in foods.
|
| 巻・号 |
361
|
| ページ |
109446
|
| 公開日 |
2022-1-16
|
| DOI |
10.1016/j.ijfoodmicro.2021.109446
|
| PII |
S0168-1605(21)00405-0
|
| PMID |
34742146
|
| MeSH |
Animals
Bacteriophages* / genetics
Chickens
Clostridium perfringens*
Meat
Milk
|
| リソース情報 |
| 一般微生物 |
JCM 1290
JCM 3816
JCM 3817
JCM 3818
JCM 3819
JCM 5242
JCM 1416
JCM 1391
JCM 1408 |
