論文 - 詳細
| RRC ID | 75293 |
|---|---|
| 著者 | Ainiwaer A, Liang Y, Ye X, Gao R. |
| タイトル | Characterization of a Novel Fe2+ Activated Non-Blue Laccase from Methylobacterium extorquens. |
| ジャーナル | Int J Mol Sci |
| Abstract |
Herein, a novel laccase gene, Melac13220, was amplified from Methylobacterium extorquens and successfully expressed in Escherichia coli with a molecular weight of approximately 50 kDa. The purified Melac13220 had no absorption peak at 610 nm and remained silent within electron paramagnetic resonance spectra, suggesting that Melac13220 belongs to the non-blue laccase group. Both inductively coupled plasma spectroscopy/optical emission spectrometry (ICP-OES) and isothermal titration calorimetry (ITC) indicated that one molecule of Melac13220 can interact with two iron ions. Furthermore, the optimal temperature of Melac13220 was 65 °C. It also showed a high thermolability, and its half-life at 65 °C was 80 min. Melac13220 showed a very good acid environment tolerance; its optimal pH was 1.5. Cu2+ and Co2+ can slightly increase enzyme activity, whereas Fe2+ could increase Melac13220's activity five-fold. Differential scanning calorimetry (DSC) indicated that Fe2+ could also stabilize Melac13220. Unlike most laccases, Melac13220 can efficiently decolorize Congo Red and Indigo Carmine dyes even in the absence of a redox mediator. Thus, the non-blue laccase from Methylobacterium extorquens shows potential application value and may be valuable for environmental protection, especially in the degradation of dyes at low pH. |
| 巻・号 | 23(17) |
| 公開日 | 2022-8-29 |
| DOI | 10.3390/ijms23179804 |
| PII | ijms23179804 |
| PMID | 36077196 |
| PMC | PMC9456135 |
| MeSH | Coloring Agents / chemistry Escherichia coli / metabolism Hydrogen-Ion Concentration Indigo Carmine Laccase* / metabolism Methylobacterium extorquens* / metabolism Temperature |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 2805 |