| RRC ID |
75455
|
| Author |
Nammali A, Duangupama T, Pittayakhajonwut P, Intaraudom C, Suriyachadkun C, He YW, Tanasupawat S, Thawai C.
|
| Title |
Streptomyces acidipaludis sp. nov., an actinobacterium isolated from peat swamp forest soil.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
An actinobacterium strain PLK6-54T was isolated from Lankwai peat swamp forest soil collected from Yala province, Thailand. Strain PLK6-54T exhibited morphological and chemotaxonomic properties described for the genus Streptomyces. It formed a spiral spore chain directly on aerial mycelium. Growth was observed between 20 and 40 °C and at pH 5-8. The maximum NaCl for growth was 2 % (w/v). ll-Diaminopimelic acid, arabinose and ribose were detected in the whole-cell hydrolysate. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside were detected as the phospholipids. The major menaquinones were MK-10(H2) and MK-9(H6). The major cellular fatty acids were iso-C16 : 0, anteiso-C15 : 0 and iso-C14 : 0. 16S rRNA gene sequence data supported the assignment of strain PLK6-54T to the genus Streptomyces and showed that Streptomyces rubidus NBRC 102073T (99.0 %) was the closest relative. Moreover, the average nucleotide identity-blast (85.5 %) and digital DNA-DNA hybridization (30.7 %) values reported between strain PLK6-54T and its closest neighbour were below the threshold values for delineation of a novel species. Strain PLK6-54T could be distinguished from related validly described Streptomyces species by several phenotypic properties. The combination of genotypic and phenotypic data indicated that strain PLK6-54T is representative of a novel species of the genus Streptomyces. The name Streptomyces acidipaludis sp. nov. is proposed for strain PLK6-54T. The type strain is PLK6-54T (=TBRC 11250T=NBRC 114297T).
|
| Volume |
72(12)
|
| Published |
2022-12-1
|
| DOI |
10.1099/ijsem.0.005626
|
| PMID |
36748585
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids* / chemistry
Forests
Phospholipids / chemistry
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil
Streptomyces*
Wetlands
|
| Resource |
| General Microbes |
JCM 12345 |
