| RRC ID |
75513
|
| 著者 |
Fujii T, Nakano M, Shinohara H, Ishikawa H, Yasutake T, Watanabe A, Funasaka K, Hirooka Y, Tochio T.
|
| タイトル |
1-Kestose Supplementation Increases Levels of a 5α-Reductase Gene, a Key Isoallolithocholic Acid Biosynthetic Gene, in the Intestinal Microbiota.
|
| ジャーナル |
J Nutr Sci Vitaminol (Tokyo)
|
| Abstract |
1-Kestose (kestose) is the smallest fructooligosaccharide component and shows a particularly high prebiotic function. Both kestose and the bile acid metabolite isoallolithocholic acid (isoalloLCA) are known to be beneficial for human health, especially in terms of immune homeostasis in the gastrointestinal system; however, the effect of kestose on the levels of microbial isoalloLCA producers remains to be clarified. IsoalloLCA is known to be produced by several members of the phylum Bacteroidota that carry the 5α-reductase (5AR) gene, a key isoalloLCA biosynthetic gene. Thus, we designed a specific primer set to detect the 5AR gene based on the consensus sequences of the genes from several isoalloLCA producers. Using real-time quantitative PCR with this primer set and fecal DNA samples, we compared the 5AR gene level (5ar-level) in the intestinal microbiota of a kestose-supplemented group (n=20) and a placebo group (n=16) before and after intake for 12 wk. The 5ar-level was significantly increased in the kestose-supplemented group (p=0.015), but not in the placebo group (p=0.379), indicating that kestose supplementation increased the 5ar-level in human intestinal microbiota. Our findings suggest that targeting functional gene levels could potentially be used to predict and understand the beneficial prebiotic effects associated with changes in gut microbiota.
|
| 巻・号 |
68(5)
|
| ページ |
446-451
|
| 公開日 |
2022-1-1
|
| DOI |
10.3177/jnsv.68.446
|
| PMID |
36310079
|
| MeSH |
Dietary Supplements
Gastrointestinal Microbiome*
Genes, vif
Humans
Oxidoreductases
Prebiotics
|
| リソース情報 |
| 一般微生物 |
JCM 13471
JCM 9497
JCM 5828 |
