Reference - Detail
| RRC ID | 75514 |
|---|---|
| Author | Saika A, Koike H, Yamamoto S, Sugahara T, Kawahara A, Sogabe A, Morita T. |
| Title | Improvement of Oil Degradation and MEL Production in a Yeast Strain, Pseudozyma tsukubaensis, by Translation Elongation Factor 1 Promoter-driven Expression of a Lipase. |
| Journal | J Oleo Sci |
| Abstract |
The basidiomycetous yeast Pseudozyma tsukubaensis produces a mannosylerythritol lipid (MEL) homologue, a diastereomer type of MEL-B, from olive oil. In a previous study, MEL-B production was increased by the overexpression of lipase PaLIPAp in P. tsukubaensis 1E5, through the enhancement of oil consumption. In the present study, RNA sequence analysis was used to identify a promoter able to induce high-level PaLIPA expression. The recombinant strain, expressing PaLIPA via the translation elongation factor 1 alpha/Tu promoter, showed higher lipase activity, rates of oil degradation, and MEL-B production than the strain which generated in our previous study. |
| Volume | 71(9) |
| Pages | 1421-1426 |
| Published | 2022-9-1 |
| DOI | 10.5650/jos.ess22089 |
| PMID | 35965089 |
| MeSH | Basidiomycota Glycolipids Lipase / genetics Lipase / metabolism Peptide Elongation Factor 1 / genetics Peptide Elongation Factor 1 / metabolism Surface-Active Agents / metabolism Ustilaginales* / genetics |
| Resource | |
| General Microbes | JCM 16987 |