論文 - 詳細
| RRC ID | 75517 |
|---|---|
| 著者 | Kinoshita Y, Kakoi H, Ishige T, Yamanaka T, Niwa H, Uchida-Fujii E, Nukada T, Ueno T. |
| タイトル | Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis. |
| ジャーナル | J Vet Med Sci |
| Abstract |
Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples. |
| 巻・号 | 84(1) |
| ページ | 129-132 |
| 公開日 | 2022-1-24 |
| DOI | 10.1292/jvms.21-0539 |
| PMID | 34853198 |
| PMC | PMC8810321 |
| MeSH | Animals Gram-Negative Bacterial Infections* / diagnosis Gram-Negative Bacterial Infections* / veterinary Horse Diseases* / diagnosis Horses Nucleic Acid Amplification Techniques / veterinary Real-Time Polymerase Chain Reaction / veterinary Taylorella equigenitalis* / genetics |
| リソース情報 | |
| 一般微生物 | JCM 9659 JCM 20522 |