| RRC ID |
75536
|
| Author |
Kang JY, Lee B, Kim JA, Kim MS, Kim CH.
|
| Title |
Identification and characterization of an ectoine biosynthesis gene cluster from Aestuariispira ectoiniformans sp. nov., isolated from seawater.
|
| Journal |
Microbiol Res
|
| Abstract |
An ectoine-producing bacterium, designated SWCN16T, was isolated from seawater and could be grown in a medium containing up to 12 % NaCl. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SWCN16T belonged to the genus Aestuariispira, class Alphaproteobacteria, and shared the highest 16S rRNA gene sequence similarity of 96.8% with Aestuariispira insulae CECT 8488T. The phenotypic, chemotaxonomic, and genotypic characteristics findings of this study suggested that strain SWCN16T represented a novel species of the genus Aestuariispira. We propose the name Aestuariispira ectoiniformans sp. nov. for this species. Whole-genome sequencing analysis of the isolate revealed a putative ectABC gene cluster for ectoine biosynthesis. These genes were found to be functional using ectoine synthesis testing and S16-ectBAC cells, which were pET21a-ectBAC-transformed E. coli BL21 cells. We found that S16-ectBAC synthesized about 1.67 g/L extracellular ectoine and about 0.59 g/L intracellular ectoine via bioconversion at optimum conditions.
|
| Volume |
254
|
| Pages |
126898
|
| Published |
2022-1-1
|
| DOI |
10.1016/j.micres.2021.126898
|
| PII |
S0944-5013(21)00204-4
|
| PMID |
34710834
|
| MeSH |
Amino Acids, Diamino* / genetics
Escherichia coli / genetics
Genes, Bacterial / genetics
Multigene Family
Phylogeny
RNA, Ribosomal, 16S / genetics
Rhodospirillaceae* / classification
Rhodospirillaceae* / genetics
Seawater* / microbiology
Species Specificity
|
| Resource |
| General Microbes |
JCM 34041 |
