| RRC ID |
75572
|
| 著者 |
Forster SC, Liu J, Kumar N, Gulliver EL, Gould JA, Escobar-Zepeda A, Mkandawire T, Pike LJ, Shao Y, Stares MD, Browne HP, Neville BA, Lawley TD.
|
| タイトル |
Strain-level characterization of broad host range mobile genetic elements transferring antibiotic resistance from the human microbiome.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Mobile genetic elements (MGEs) carrying antibiotic resistance genes (ARGs) disseminate ARGs when they mobilise into new bacterial hosts. The nature of such horizontal gene transfer (HGT) events between human gut commensals and pathogens remain poorly characterised. Here, we compare 1354 cultured commensal strains (540 species) to 45,403 pathogen strains (12 species) and find 64,188 MGE-mediated ARG transfer events between the two groups using established methods. Among the 5931 MGEs, we find 15 broad host range elements predicted to have crossed different bacterial phyla while also occurring in animal and environmental microbiomes. We experimentally demonstrate that predicted broad host range MGEs can mobilise from commensals Dorea longicatena and Hungatella hathewayi to pathogen Klebsiella oxytoca, crossing phyla simultaneously. Our work establishes the MGE-mediated ARG dissemination network between human gut commensals and pathogens and highlights broad host range MGEs as targets for future ARG dissemination management.
|
| 巻・号 |
13(1)
|
| ページ |
1445
|
| 公開日 |
2022-3-17
|
| DOI |
10.1038/s41467-022-29096-9
|
| PII |
10.1038/s41467-022-29096-9
|
| PMID |
35301310
|
| PMC |
PMC8931123
|
| MeSH |
Animals
Anti-Bacterial Agents / pharmacology
Bacteria / genetics
Drug Resistance, Microbial / genetics
Genes, Bacterial
Host Specificity* / genetics
Humans
Interspersed Repetitive Sequences / genetics
Microbiota* / genetics
|
| リソース情報 |
| 一般微生物 |
JCM 31258 |
