RRC ID 75589
著者 Klangpetch W, Pattarapisitporn A, Phongthai S, Utama-Ang N, Laokuldilok T, Tangjaidee P, Wirjantoro TI, Jaichakan P.
タイトル Microwave-assisted enzymatic hydrolysis to produce xylooligosaccharides from rice husk alkali-soluble arabinoxylan.
ジャーナル Sci Rep
Abstract The prebiotic properties of xylooligosaccharides (XOS) and arabino-xylooligosaccharides (AXOS) produced from rice husk (RH) using microwave treatment combined with enzymatic hydrolysis were evaluated. The RH was subjected to microwave pretreatment at 140, 160 and 180 °C for 5, 10 and 15 min to obtain crude arabinoxylan (AX). Increasing microwave pretreatment time increased sugar content. Crude AX was extracted with 2% (w/v) sodium hydroxide at 25 °C for 24 h and used as a substrate for XOS production by commercial xylanases. Results showed that oligosaccharides produced by Pentopan Mono BG and Ultraflo Max provided xylobiose and xylotriose as the main products. AXOS was also present in the oligosaccharides that promoted growth of Lactobacillus spp. and resisted degradation by over 70% after exposure to simulated human digestion.
巻・号 12(1)
ページ 11
公開日 2022-1-7
DOI 10.1038/s41598-021-03360-2
PII 10.1038/s41598-021-03360-2
PMID 34996923
PMC PMC8741828
MeSH Alkalies / chemistry Disaccharides / analysis Endo-1,4-beta Xylanases / chemistry* Glucuronates / chemistry* Hydrolysis Microwaves Oligosaccharides / chemistry* Oryza / chemistry* Oryza / radiation effects Prebiotics / analysis Seeds / chemistry Trisaccharides / analysis Xylans / chemistry*
リソース情報
一般微生物 JCM 1149 JCM 1157 JCM 1002