| RRC ID |
75592
|
| Author |
Yabuuchi S, Oiki S, Minami S, Takase R, Watanabe D, Hashimoto W.
|
| Title |
Enhanced propagation of Granulicatella adiacens from human oral microbiota by hyaluronan.
|
| Journal |
Sci Rep
|
| Abstract |
Host determinants for formation/composition of human oral microbiota remain to be clarified, although microorganisms entering the mouth cannot necessarily colonize the oral environment. Here we show that human oral-abundant bacteria degraded host glycosaminoglycans (GAGs) in saliva and gingiva, and certain bacteria significantly grew on hyaluronan (HA), a kind of GAGs. Microbial communities from teeth or gingiva of healthy donors assimilated HA. Metagenomic analysis of human oral microbiota under different carbon sources revealed HA-driven Granulicatella growth. HA-degrading bacterial strains independently isolated from teeth and gingiva were identified as Granulicatella adiacens producing extracellular 130 kDa polysaccharide lyase as a HA-degrading enzyme encoded in a peculiar GAG genetic cluster containing genes for isomerase KduI and dehydrogenase DhuD. These findings demonstrated that GAGs are one of the host determinants for formation/composition of oral microbiota not only for colonization but also for the adaptation to the host niche. Especially, HA enhanced the G. adiacens propagation.
|
| Volume |
12(1)
|
| Pages |
10948
|
| Published |
2022-6-29
|
| DOI |
10.1038/s41598-022-14857-9
|
| PII |
10.1038/s41598-022-14857-9
|
| PMID |
35768476
|
| PMC |
PMC9243090
|
| MeSH |
Bacteria / metabolism
Carnobacteriaceae* / metabolism
Glycosaminoglycans / metabolism
Humans
Hyaluronic Acid / metabolism
Microbiota*
Streptococcus / metabolism
|
| Resource |
| General Microbes |
JCM 16131
JCM 1320
JCM 12992
JCM 13448
JCM 16774
JCM 12997
JCM 8152 |
