| RRC ID |
75594
|
| 著者 |
Aoki H, Masahiro Y, Shimizu M, Hongoh Y, Ohkuma M, Yamagata Y.
|
| タイトル |
Agarose gel microcapsules enable easy-to-prepare, picolitre-scale, single-cell genomics, yielding high-coverage genome sequences.
|
| ジャーナル |
Sci Rep
|
| Abstract |
A novel type of agarose gel microcapsule (AGM), consisting of an alginate picolitre sol core and an agarose gel shell, was developed to obtain high-quality, single-cell, amplified genomic DNA of bacteria. The AGM is easy to prepare in a stable emulsion with oil of water-equivalent density, which prevents AGM aggregation, with only standard laboratory equipment. Single cells from a pure culture of Escherichia coli, a mock community comprising 15 strains of human gut bacteria, and a termite gut bacterial community were encapsulated within AGMs, and their genomic DNA samples were prepared with massively parallel amplifications in a tube. The genome sequencing did not need second-round amplification and showed an average genome completeness that was much higher than that obtained using a conventional amplification method on the microlitre scale, regardless of the genomic guanine-cytosine content. Our novel method using AGM will allow many researchers to perform single-cell genomics easily and effectively, and can accelerate genomic analysis of yet-uncultured microorganisms.
|
| 巻・号 |
12(1)
|
| ページ |
17014
|
| 公開日 |
2022-10-18
|
| DOI |
10.1038/s41598-022-20923-z
|
| PII |
10.1038/s41598-022-20923-z
|
| PMID |
36257967
|
| PMC |
PMC9579161
|
| MeSH |
Alginates
Bacteria* / genetics
Capsules
Cytosine
DNA
Emulsions
Genome, Bacterial
Genomics* / methods
Guanine
Humans
Sepharose
Water
|
| リソース情報 |
| 一般微生物 |
JCM 5827
JCM 1471
JCM 10609
JCM 12243
JCM 10188
JCM 31915
JCM 32125
JCM 14723
JCM 14723
JCM 5825
JCM 13464
JCM 17581
JCM 6515
JCM 5705
JCM 17976 |
