論文 - 詳細
| RRC ID | 75687 |
|---|---|
| 著者 | Hamajima R, Lusiany T, Minami S, Nouda R, Nurdin JA, Yamasaki M, Kobayashi N, Kanai Y, Kobayashi T. |
| タイトル | A reverse genetics system for human rotavirus G2P[4]. |
| ジャーナル | J Gen Virol |
| Abstract |
Rotaviruses (RVs) are an important cause of acute gastroenteritis in young children. Recently, versatile plasmid-based reverse genetics systems were developed for several human RV genotypes; however, these systems have not been developed for all commonly circulating human RV genotypes. In this study, we established a reverse genetics system for G2P[4] human RV strain HN126. Nucleotide sequence analysis, including that of the terminal ends of the viral double-stranded RNA genome, revealed that HN126 possessed a DS-1-like genotype constellation. Eleven plasmids, each encoding 11 gene segments of the RV genome, and expression plasmids encoding vaccinia virus RNA capping enzyme (D1R and D12L), Nelson Bay orthoreovirus FAST, and NSP2 and NSP5 of HN126, were transfected into BHK-T7 cells, and recombinant strain HN126 was generated. Using HN126 or simian RV strain SA11 as backbone viruses, reassortant RVs carrying the outer and intermediate capsid proteins (VP4, VP7 and VP6) of HN126 and/or SA11 (in various combinations) were generated. Viral replication analysis of the single, double and triple reassortant viruses suggested that homologous combination of the VP4 and VP7 proteins contributed to efficient virus infectivity and interaction between other viral or cellular proteins. Further studies of reassortant viruses between simian and other human RV strains will contribute to developing an appropriate model for human RV research, as well as suitable backbone viruses for generation of recombinant vaccine candidates. |
| 巻・号 | 103(12) |
| 公開日 | 2022-12-1 |
| DOI | 10.1099/jgv.0.001816 |
| PMID | 36748482 |
| MeSH | Genome, Viral* Genotype Humans Phylogeny Reassortant Viruses / genetics Reverse Genetics Rotavirus* / genetics |
| IF | 3.376 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト病原ウイルス | G2P[4] human RV strain HN126 |