RRC ID 75729
Author Watanabe K, Oka T, Takagi H, Anisimov S, Yamashita SI, Katsuragi Y, Takahashi M, Higuchi M, Kanki T, Saitoh A, Fujii M.
Title Myeloid-associated differentiation marker is an essential host factor for human parechovirus PeV-A3 entry.
Journal Nat Commun
Abstract Human parechovirus (PeV-A) is an RNA virus that belongs to the family Picornaviridae and it is currently classified into 19 genotypes. PeV-As usually cause mild illness in children and adults. Among the genotypes, PeV-A3 can cause severe diseases in neonates and young infants, resulting in neurological sequelae and death. In this study, we identify the human myeloid-associated differentiation marker (MYADM) as an essential host factor for the entry of six PeV-As (PeV-A1 to PeV-A6), including PeV-A3. The infection of six PeV-As (PeV-A1 to PeV-A6) to human cells is abolished by knocking out the expression of MYADM. Hamster BHK-21 cells are resistant to PeV-A infection, but the expression of human MYADM in BHK-21 confers PeV-A infection and viral production. Furthermore, VP0 capsid protein of PeV-A3 interacts with one extracellular domain of human MYADM on the cell membrane of BHK-21. The identification of MYADM as an essential entry factor for PeV-As infection is expected to advance our understanding of the pathogenesis of PeV-As.
Volume 14(1)
Pages 1817
Published 2023-3-31
DOI 10.1038/s41467-023-37399-8
PII 10.1038/s41467-023-37399-8
PMID 37002207
PMC PMC10066301
MeSH Adult Antigens, Differentiation Child Genotype Humans Infant Infant, Newborn Parechovirus* / genetics Picornaviridae* Picornaviridae Infections*
IF 12.121
DNA material CSII-EF-MCS (RDB04378) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394)