| RRC ID |
75760
|
| Author |
Kasayama S, Sumitani S, Tanaka A, Yamanishi H, Nakamura N, Matsumoto K, Sato B.
|
| Title |
Heparin inhibits autocrine stimulation but not fibroblast growth factor stimulation of cell proliferation of androgen-responsive Shionogi carcinoma 115.
|
| Journal |
J Cell Physiol
|
| Abstract |
An androgen-responsive cloned cell line (SC-3) derived from Shionogi carcinoma 115 (SC115) has been shown to secrete fibroblast growth factor (FGF)-like peptide in response to androgen, which binds to FGF receptor and promotes the proliferation of SC-3 cells in an autocrine mechanism. Since the androgen-induced autocrine factor has a property to bind heparin, we examined the effects of heparin on the growth of SC-3 cells. Heparin was found to exhibit significant inhibition of testosterone-induced growth in a concentration-dependent manner: Approximately 50% inhibition was found at a concentration of 0.1 micrograms/ml. DNA synthesis of SC-3 cells induced by testosterone was also inhibited strongly by heparin, and less strongly by heparan sulfate and dermatan sulfate. Proliferation of SC-3 cells induced by acidic (a) or basic (b) FGF appeared not to be modulated by heparin. In contrast, heparin efficiently blocked DNA synthesis stimulated with androgen-induced growth factor in the conditioned medium from testosterone-treated cells. These results indicate that heparin inhibits autocrine loop in SC-3 cells induced by androgen. Thus, the autocrine growth factor possesses a different characteristic from aFGF and bFGF in that its bioactivities are negatively modulated by the glycosaminoglycan.
|
| Volume |
148(2)
|
| Pages |
260-6
|
| Published |
1991-8-1
|
| DOI |
10.1002/jcp.1041480211
|
| PMID |
1715343
|
| MeSH |
Animals
Cell Division / drug effects*
Cell Line
Culture Media
DNA, Neoplasm / biosynthesis
Fibroblast Growth Factor 1 / pharmacology
Fibroblast Growth Factor 2 / pharmacology
Fibroblast Growth Factors / pharmacology*
Growth Substances / pharmacology*
Heparin / pharmacology*
Testosterone / pharmacology*
Tumor Cells, Cultured
|
| IF |
5.546
|
| Resource |
| Human and Animal Cells |
NIH3T3-3(RCB0150) |
