| RRC ID |
75777
|
| 著者 |
Kozaki K, Miyaishi O, Asai N, Iida K, Sakata K, Hayashi M, Nishida T, Matsuyama M, Shimizu S, Kaneda T.
|
| タイトル |
Tissue distribution of ERp61 and association of its increased expression with IgG production in hybridoma cells.
|
| ジャーナル |
Exp Cell Res
|
| Abstract |
A protein of molecular weight 60 kDa was purified from the culture medium of a murine colon carcinoma cell line, colon26, and its partial amino-acid sequence determined. Extremely high homology was found with the deduced sequence from cDNA of rat ERp61, earlier found to be an endoplasmic reticulum (ER)-resident protein with redox activity and a similar structure to protein disulfide isomerase (PDI). Western blotting analysis showed that colon26 cells secrete a significant amount of ERp61 into culture medium, although most remains intracellular. The thiol:protein disulfide oxidoreductase activity of the purified mouse ERp61 was demonstrated by insulin-reduction assay. The ER location of the protein in fibroblasts was immunocytochemically confirmed by double staining for ERp61 and another ER-resident protein, PDI or Hsp47. Immunohistochemical studies of murine tissues showed a ubiquitous distribution of ERp61 in a wide variety of cell types. However, it was particularly abundant in plasma cells, mucus-secreting cells in various tissues, neuroendocrine cells including neurons, and follicular epithelia of thyroid gland that actively synthesize and secrete proteins containing cysteine residues. Furthermore, a high correlation was observed between intracellular amounts of ERp61 and immunoglobulin production by hybridoma cells. These results indicate that ERp61 may be involved in disulfide bond formation for such proteins.
|
| 巻・号 |
213(2)
|
| ページ |
348-58
|
| 公開日 |
1994-8-1
|
| DOI |
10.1006/excr.1994.1209
|
| PII |
S0014482784712092
|
| PMID |
8050492
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Blotting, Western
Colonic Neoplasms
DNA, Neoplasm
Endoplasmic Reticulum / immunology
Endoplasmic Reticulum / metabolism*
Heat-Shock Proteins / immunology
Heat-Shock Proteins / metabolism*
Hybridomas / cytology
Hybridomas / immunology
Immunoglobulin G / biosynthesis*
Immunohistochemistry
Isomerases*
Mice
Molecular Sequence Data
Organ Specificity
Oxidation-Reduction
Protein Disulfide-Isomerases
Tumor Cells, Cultured
|
| IF |
3.383
|
| リソース情報 |
| ヒト・動物細胞 |
SP2/0-Ag14(RCB0209) |
