論文 - 詳細
| RRC ID | 75777 |
|---|---|
| 著者 | Kozaki K, Miyaishi O, Asai N, Iida K, Sakata K, Hayashi M, Nishida T, Matsuyama M, Shimizu S, Kaneda T. |
| タイトル | Tissue distribution of ERp61 and association of its increased expression with IgG production in hybridoma cells. |
| ジャーナル | Exp Cell Res |
| Abstract |
A protein of molecular weight 60 kDa was purified from the culture medium of a murine colon carcinoma cell line, colon26, and its partial amino-acid sequence determined. Extremely high homology was found with the deduced sequence from cDNA of rat ERp61, earlier found to be an endoplasmic reticulum (ER)-resident protein with redox activity and a similar structure to protein disulfide isomerase (PDI). Western blotting analysis showed that colon26 cells secrete a significant amount of ERp61 into culture medium, although most remains intracellular. The thiol:protein disulfide oxidoreductase activity of the purified mouse ERp61 was demonstrated by insulin-reduction assay. The ER location of the protein in fibroblasts was immunocytochemically confirmed by double staining for ERp61 and another ER-resident protein, PDI or Hsp47. Immunohistochemical studies of murine tissues showed a ubiquitous distribution of ERp61 in a wide variety of cell types. However, it was particularly abundant in plasma cells, mucus-secreting cells in various tissues, neuroendocrine cells including neurons, and follicular epithelia of thyroid gland that actively synthesize and secrete proteins containing cysteine residues. Furthermore, a high correlation was observed between intracellular amounts of ERp61 and immunoglobulin production by hybridoma cells. These results indicate that ERp61 may be involved in disulfide bond formation for such proteins. |
| 巻・号 | 213(2) |
| ページ | 348-58 |
| 公開日 | 1994-8-1 |
| DOI | 10.1006/excr.1994.1209 |
| PII | S0014482784712092 |
| PMID | 8050492 |
| MeSH | Amino Acid Sequence Animals Base Sequence Blotting, Western Colonic Neoplasms DNA, Neoplasm Endoplasmic Reticulum / immunology Endoplasmic Reticulum / metabolism* Heat-Shock Proteins / immunology Heat-Shock Proteins / metabolism* Hybridomas / cytology Hybridomas / immunology Immunoglobulin G / biosynthesis* Immunohistochemistry Isomerases* Mice Molecular Sequence Data Organ Specificity Oxidation-Reduction Protein Disulfide-Isomerases Tumor Cells, Cultured |
| IF | 3.383 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | SP2/0-Ag14(RCB0209) |