論文 - 詳細
| RRC ID | 75778 |
|---|---|
| 著者 | Sawaoka H, Tsuji S, Tsujii M, Gunawan ES, Nakama A, Takei Y, Nagano K, Matsui H, Kawano S, Hori M. |
| タイトル | Expression of the cyclooxygenase-2 gene in gastric epithelium. |
| ジャーナル | J Clin Gastroenterol |
| Abstract |
This study examined the mRNA level of cyclooxygenase-2 (cox-2) in a rat gastric mucosal cell line after growth stimulation in vitro and in rat gastric mucosa before and after acid-induced injury in vivo. RGMI cells were stimulated with fetal calf serum in the in vitro study. Thereafter, the cox-2 mRNA level was examined by Northern analysis. Effects of NS-398, a specific inhibitor of cox-2, and indomethacin on prostaglandin production and cell proliferation were examined in RGM1 cells. In the in vivo study, rats were given 1 ml of 0.6 N hydrochloric acid into the stomach. The level of cox-2 mRNA was examined in rat gastric mucosa after acid administration. Cox-2 mRNA increased 20-60 min after growth stimulation in RGM1 cells. Both NS-398 and indomethacin suppressed prostaglandin production and cell proliferation after growth stimulation. Expression of cox-2 mRNA was observed 40 and 60 min after administration of 0.6 N HCl. Gastric lesions developed within 60 min after HCl administration and healed significantly within 48 h. The present study shows the expression of cox-2 in gastric epithelial cells in vitro and in gastric mucosa after injury in vivo. The results also suggest that cox-2 is involved in de novo synthesis of prostaglandins and cell proliferation in gastric epithelium. |
| 巻・号 | 25 Suppl 1 |
| ページ | S105-10 |
| 公開日 | 1997-1-1 |
| DOI | 10.1097/00004836-199700001-00018 |
| PMID | 9479635 |
| MeSH | Animals Cell Line Cyclooxygenase 2 Cyclooxygenase 2 Inhibitors Cyclooxygenase Inhibitors / pharmacology Gastric Mucosa / cytology Gastric Mucosa / drug effects Gastric Mucosa / enzymology* Gene Expression Hydrochloric Acid Indomethacin / pharmacology Isoenzymes / biosynthesis* Isoenzymes / genetics Male Nitrobenzenes / pharmacology Peroxidases / biosynthesis* Peroxidases / genetics Prostaglandin-Endoperoxide Synthases / biosynthesis* Prostaglandin-Endoperoxide Synthases / genetics RNA, Messenger / genetics Rats Rats, Sprague-Dawley Sulfonamides / pharmacology Time Factors |
| IF | 3.899 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |