| RRC ID |
75778
|
| Author |
Sawaoka H, Tsuji S, Tsujii M, Gunawan ES, Nakama A, Takei Y, Nagano K, Matsui H, Kawano S, Hori M.
|
| Title |
Expression of the cyclooxygenase-2 gene in gastric epithelium.
|
| Journal |
J Clin Gastroenterol
|
| Abstract |
This study examined the mRNA level of cyclooxygenase-2 (cox-2) in a rat gastric mucosal cell line after growth stimulation in vitro and in rat gastric mucosa before and after acid-induced injury in vivo. RGMI cells were stimulated with fetal calf serum in the in vitro study. Thereafter, the cox-2 mRNA level was examined by Northern analysis. Effects of NS-398, a specific inhibitor of cox-2, and indomethacin on prostaglandin production and cell proliferation were examined in RGM1 cells. In the in vivo study, rats were given 1 ml of 0.6 N hydrochloric acid into the stomach. The level of cox-2 mRNA was examined in rat gastric mucosa after acid administration. Cox-2 mRNA increased 20-60 min after growth stimulation in RGM1 cells. Both NS-398 and indomethacin suppressed prostaglandin production and cell proliferation after growth stimulation. Expression of cox-2 mRNA was observed 40 and 60 min after administration of 0.6 N HCl. Gastric lesions developed within 60 min after HCl administration and healed significantly within 48 h. The present study shows the expression of cox-2 in gastric epithelial cells in vitro and in gastric mucosa after injury in vivo. The results also suggest that cox-2 is involved in de novo synthesis of prostaglandins and cell proliferation in gastric epithelium.
|
| Volume |
25 Suppl 1
|
| Pages |
S105-10
|
| Published |
1997-1-1
|
| DOI |
10.1097/00004836-199700001-00018
|
| PMID |
9479635
|
| MeSH |
Animals
Cell Line
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Cyclooxygenase Inhibitors / pharmacology
Gastric Mucosa / cytology
Gastric Mucosa / drug effects
Gastric Mucosa / enzymology*
Gene Expression
Hydrochloric Acid
Indomethacin / pharmacology
Isoenzymes / biosynthesis*
Isoenzymes / genetics
Male
Nitrobenzenes / pharmacology
Peroxidases / biosynthesis*
Peroxidases / genetics
Prostaglandin-Endoperoxide Synthases / biosynthesis*
Prostaglandin-Endoperoxide Synthases / genetics
RNA, Messenger / genetics
Rats
Rats, Sprague-Dawley
Sulfonamides / pharmacology
Time Factors
|
| IF |
3.899
|
| Resource |
| Human and Animal Cells |
RGM1(RCB0876) |
