| RRC ID |
75779
|
| Author |
Kasayama S, Koga M, Kouhara H, Sumitani S, Wada K, Kishimoto T, Sato B.
|
| Title |
Unsaturated fatty acids are required for continuous proliferation of transformed androgen-dependent cells by fibroblast growth factor family proteins.
|
| Journal |
Cancer Res
|
| Abstract |
Increase in dietary fat intake has been reported to be associated with progression of hormone-dependent cancers. To explore its mechanism, we examined the effects of fatty acids on the growth of androgen-dependent SC-3 cells cloned from mouse mammary cancer (Shionogi carcinoma 115). Their androgen-dependent growth was potentiated by linoleic acid in the defined medium. The effect of linoleic acid on fibroblast growth factor (FGF)-dependent growth was also addressed because androgen had been demonstrated to exert its mitogenic activity on SC-3 cells through an induction of the unique FGF family protein termed as androgen-induced growth factor. Exposure of SC-3 cells to basic FGF or androgen-induced growth factor exhibited only transient growth response. However, simultaneous addition of linoleic acid to the medium sustained the proliferation of FGF-stimulated, but not FGF-unstimulated, cells, although linoleic acid did not exert the significant effect on the process of S-phase entry of basic FGF-stimulated cells. Palmitoleic acid and oleic acid appeared to exert the actions similar to linoleic acid, while stearic acid was without any effect. Neither cyclooxygenase inhibitor nor 5-lipoxygenase inhibitor could block the growth-promoting ability of linoleic acid. Linoleic acid also enhanced their anchorage-independent growth in the presence of basic FGF. These results indicate that these unsaturated fatty acids play a role in sustaining the proliferation of FGF-stimulated SC-3 cells.
|
| Volume |
54(24)
|
| Pages |
6441-5
|
| Published |
1994-12-15
|
| PMID |
7987840
|
| MeSH |
Animals
Arachidonic Acids / pharmacology
Benzoquinones / pharmacology
Cell Adhesion
Cell Cycle / drug effects
Cell Division / drug effects
Culture Media, Serum-Free
Fatty Acids / pharmacology*
Fatty Acids, Monounsaturated / pharmacology
Fibroblast Growth Factor 2 / pharmacology
Indomethacin / pharmacology
Linoleic Acid
Linoleic Acids / antagonists & inhibitors
Linoleic Acids / pharmacology
Mammary Neoplasms, Animal / pathology*
Mice
Neoplasms, Hormone-Dependent / pathology*
Oleic Acid
Oleic Acids / pharmacology
Testosterone / pharmacology
Tumor Cells, Cultured
|
| IF |
9.727
|
| Resource |
| Human and Animal Cells |
NIH3T3-3(RCB0150) |
