Reference - Detail
| RRC ID | 75780 |
|---|---|
| Author | Wakimoto H, Abe J, Tsunoda R, Aoyagi M, Hirakawa K, Hamada H. |
| Title | Intensified antitumor immunity by a cancer vaccine that produces granulocyte-macrophage colony-stimulating factor plus interleukin 4. |
| Journal | Cancer Res |
| Abstract |
Vaccination with irradiated tumor cells genetically modified to secrete granulocyte-macrophage colony-stimulating factor (GM-CSF tumor vaccine) induces a potent systemic antitumor immunity. To develop a protocol for cancer therapy to further augment the host immune response, we examined the effects of the GM-CSF tumor vaccines simultaneously producing additional cytokines. We prepared cancer vaccines expressing double cytokines by sequential recombinant retrovirus-mediated genetic transductions. We then used a murine intracerebral tumor model in which the GM-CSF tumor vaccine was less effective in immunopotentiation and evaluated tumor vaccines producing various cytokines in conjunction with GM-CSF. The cytokine combination of GM-CSF and interleukin 4 induced more potent antitumor immunity than GM-CSF alone. An in vivo depletion test showed that CD4+, CD8+, and asialoGM1+ cells were required for the optimum function of the GM-CSF plus interleukin 4 tumor vaccine. Histological examinations revealed infiltration of inflammatory cells at the site of tumor cell challenge as well as at the site of vaccination, indicating the induction of a systemic antitumor immune response which reached the central nervous system. Our findings suggest the feasibility of applying the intensified vaccination strategy to treat human cancers including malignant brain tumors. |
| Volume | 56(8) |
| Pages | 1828-33 |
| Published | 1996-4-15 |
| PMID | 8620500 |
| MeSH | 3T3 Cells Animals Brain Neoplasms / immunology Brain Neoplasms / pathology Brain Neoplasms / secondary Brain Neoplasms / therapy* Cytokines / biosynthesis Female Genetic Vectors Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis* Humans Immunotherapy* Interleukin-4 / biosynthesis* Melanoma, Experimental / immunology Melanoma, Experimental / pathology Melanoma, Experimental / secondary* Melanoma, Experimental / therapy Mice Mice, Inbred C57BL Polymerase Chain Reaction Retroviridae Vaccines, Synthetic* |
| IF | 9.727 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 18 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pMFGmGM-CSF (RDB01438) pMFGmIL2 (RDB01441) pMFGmIL3 (RDB01442) pMFGmIL4 (RDB01443) pMFGmIL6 (RDB01444) pMFGmIL7 (RDB01445) pMFGmTNF alpha (RDB01449) pMFGmLT (RDB01451) pMFGmG-CSF (RDB01452) pMFGmLIF (RDB01456) |