| RRC ID |
75841
|
| 著者 |
Umezawa T, Okamoto M, Kushiro T, Nambara E, Oono Y, Seki M, Kobayashi M, Koshiba T, Kamiya Y, Shinozaki K.
|
| タイトル |
CYP707A3, a major ABA 8'-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana.
|
| ジャーナル |
Plant J
|
| Abstract |
Abscisic acid (ABA) catabolism is one of the determinants of endogenous ABA levels affecting numerous aspects of plant growth and abiotic stress responses. The major ABA catabolic pathway is triggered by ABA 8'-hydroxylation catalysed by the cytochrome P450 CYP707A family. Among four members of Arabidopsis CYP707As, the expression of CYP707A3 was most highly induced in response to both dehydration and subsequent rehydration. A T-DNA insertional cyp707a3-1 mutant contained higher ABA levels in turgid plants, which showed a reduced transpiration rate and hypersensitivity to exogenous ABA during early seedling growth. On dehydration, the cyp707a3-1 mutant accumulated a higher amount of stress-induced ABA than the wild type, an event that occurred relatively later and was coincident with slow drought induction of CYP707A3. The cyp707a3 mutant plants exhibited both exaggerated ABA-inducible gene expression and enhanced drought tolerance. Conversely, constitutive expression of CYP707A3 relieved growth retardation by ABA, increased transpiration, and a reduction of endogenous ABA in both turgid and dehydrated plants. Taken together, our results indicate that CYP707A3 plays an important role in determining threshold levels of ABA during dehydration and after rehydration.
|
| 巻・号 |
46(2)
|
| ページ |
171-82
|
| 公開日 |
2006-4-1
|
| DOI |
10.1111/j.1365-313X.2006.02683.x
|
| PII |
TPJ2683
|
| PMID |
16623881
|
| MeSH |
Abscisic Acid / physiology
Arabidopsis / enzymology*
Arabidopsis / genetics
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Cytochrome P-450 Enzyme System / genetics*
Cytochrome P-450 Enzyme System / metabolism
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Mixed Function Oxygenases / genetics*
Mixed Function Oxygenases / metabolism
Mutagenesis, Insertional
Plant Proteins
RNA, Plant / genetics
RNA, Plant / isolation & purification
Signal Transduction
Transcription, Genetic
Water
|
| IF |
6.141
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda05432 |
