論文 - 詳細
| RRC ID | 75848 |
|---|---|
| 著者 | Kuromiya K, Aoki K, Ishibashi K, Yotabun M, Sekai M, Tanimura N, Iijima S, Ishikawa S, Kamasaki T, Akieda Y, Ishitani T, Hayashi T, Toda S, Yokoyama K, Lee CG, Usami I, Inoue H, Takigawa I, Gauquelin E, Sugimura K, Hino N, Fujita Y. |
| タイトル | Calcium sparks enhance the tissue fluidity within epithelial layers and promote apical extrusion of transformed cells. |
| ジャーナル | Cell Rep |
| Abstract |
In vertebrates, newly emerging transformed cells are often apically extruded from epithelial layers through cell competition with surrounding normal epithelial cells. However, the underlying molecular mechanism remains elusive. Here, using phospho-SILAC screening, we show that phosphorylation of AHNAK2 is elevated in normal cells neighboring RasV12 cells soon after the induction of RasV12 expression, which is mediated by calcium-dependent protein kinase C. In addition, transient upsurges of intracellular calcium, which we call calcium sparks, frequently occur in normal cells neighboring RasV12 cells, which are mediated by mechanosensitive calcium channel TRPC1 upon membrane stretching. Calcium sparks then enhance cell movements of both normal and RasV12 cells through phosphorylation of AHNAK2 and promote apical extrusion. Moreover, comparable calcium sparks positively regulate apical extrusion of RasV12-transformed cells in zebrafish larvae as well. Hence, calcium sparks play a crucial role in the elimination of transformed cells at the early phase of cell competition. |
| 巻・号 | 40(2) |
| ページ | 111078 |
| 公開日 | 2022-7-12 |
| DOI | 10.1016/j.celrep.2022.111078 |
| PII | S2211-1247(22)00876-2 |
| PMID | 35830802 |
| MeSH | Animals Calcium / metabolism Calcium Signaling* Cell Movement Dogs Epithelial Cells / metabolism Madin Darby Canine Kidney Cells Zebrafish* / metabolism |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 21 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Krt4p:Gal4 UAS:GFP |