Reference - Detail
| RRC ID | 75855 |
|---|---|
| Author | Shkarina K, Hasel de Carvalho E, Santos JC, Ramos S, Leptin M, Broz P. |
| Title | Optogenetic activators of apoptosis, necroptosis, and pyroptosis. |
| Journal | J Cell Biol |
| Abstract |
Targeted and specific induction of cell death in an individual or groups of cells hold the potential for new insights into the response of tissues or organisms to different forms of death. Here, we report the development of optogenetically controlled cell death effectors (optoCDEs), a novel class of optogenetic tools that enables light-mediated induction of three types of programmed cell death (PCD)-apoptosis, pyroptosis, and necroptosis-using Arabidopsis thaliana photosensitive protein Cryptochrome-2. OptoCDEs enable a rapid and highly specific induction of PCD in human, mouse, and zebrafish cells and are suitable for a wide range of applications, such as sub-lethal cell death induction or precise elimination of single cells or cell populations in vitro and in vivo. As the proof-of-concept, we utilize optoCDEs to assess the differences in neighboring cell responses to apoptotic or necrotic PCD, revealing a new role for shingosine-1-phosphate signaling in regulating the efferocytosis of the apoptotic cell by epithelia. |
| Volume | 221(6) |
| Published | 2022-6-6 |
| DOI | 10.1083/jcb.202109038 |
| PII | 213145 |
| PMID | 35420640 |
| PMC | PMC9014795 |
| MeSH | Animals Apoptosis* / genetics Arabidopsis / genetics Cryptochromes / genetics Humans Lysophospholipids / metabolism Mice Necroptosis* / genetics Optogenetics* Pyroptosis* / genetics Sphingosine / analogs & derivatives Sphingosine / metabolism Zebrafish / genetics |
| IF | 8.811 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 204 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Zebrafish | Krt4p:Gal4 |