| RRC ID |
75898
|
| 著者 |
Hata Ji, Takeo J, Segawa C, Yamashita S.
|
| タイトル |
A cDNA encoding fish fibroblast growth factor-2, which lacks alternative translation initiation.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Here, we describe the isolation of a rainbow trout cDNA clone that contains the entire fibroblast growth factor-2 (FGF-2; basic FGF) coding region. Interestingly, the rainbow trout cDNA contains a translation stop codon just upstream of the primary initiating methionine codon and so cannot give rise to the longer forms of FGF-2 that are produced in mammals by alternative translation initiation at leucines farther upstream. Transfection of human FGF-2 cDNA into fish cells shows that fish cells can initiate protein synthesis at an upstream leucine CUG codon; surprisingly, however, synthesis is initiated only at the most 5' CUG and not at the two subsequent CUG codons or the methionine AUG codon also used in mammalian cells. Like other FGF-2 proteins, bacterially produced rainbow trout FGF-2 protein binds tightly to heparin-Sepharose and also promotes the proliferation of fibroblast cells. However, the protein differs from all others previously identified at amino acids 121-123, which are part of the proposed highly conserved receptor-binding domain. Comparisons of the efficacies of recombinant wild-type and mutant rainbow trout FGF-2 proteins demonstrate that these three amino acids are critical to the activity of FGF-2.
|
| 巻・号 |
272(11)
|
| ページ |
7285-9
|
| 公開日 |
1997-3-14
|
| DOI |
10.1074/jbc.272.11.7285
|
| PII |
S0021-9258(18)41066-6
|
| PMID |
9054425
|
| MeSH |
Amino Acid Sequence
Animals
Cloning, Molecular
DNA, Complementary / genetics
DNA, Complementary / isolation & purification
Fibroblast Growth Factor 2 / genetics*
Fibroblast Growth Factor 2 / isolation & purification
Humans
Molecular Sequence Data
Oncorhynchus mykiss
Peptide Chain Initiation, Translational / genetics*
Protein Biosynthesis
Sequence Alignment
|
| IF |
4.238
|
| リソース情報 |
| ヒト・動物細胞 |
BALB/3T3 clone A31(RCB0005)
COS-7(RCB0539) |
