RRC ID 75899
Author Ebara S, Kawasaki S, Nakamura I, Tsutsumimoto T, Nakayama K, Nikaido T, Takaoka K.
Title Transcriptional regulation of the mBMP-4 gene through an E-box in the 5'-flanking promoter region involving USF.
Journal Biochem Biophys Res Commun
Abstract We analyzed the promoter activity of murine bone morphogenetic protein-4 (mBMP-4) and determined that the 5'-flanking region of exon I plays a critical role for transcription and position -265 to -246 of 5'-flanking region of mBMP-4 gene acts as a cis-element important for the regulation of BMP4 transcription in MC3T3E1 cells. By use of site-directed mutagenesis, we have established that the E-box, CACGTG, located within this short region of promoter, is essential for transcriptional activation of the mBMP-4 gene. Upstream stimulatory factor (USF), a member of the helix-loop-helix (HLH) group of regulatory proteins, was found to bind to this E-box using supershift gel mobility analysis. It is proposed that the HLH transcription regulatory proteins play an important role in mBMP-4 gene transcription in this osteoblastic cell line.
Volume 240(1)
Pages 136-41
Published 1997-11-7
DOI 10.1006/bbrc.1997.7618
PII S0006-291X(97)97618-X
PMID 9367898
MeSH Animals Binding, Competitive / genetics Bone Morphogenetic Protein 4 Bone Morphogenetic Proteins / genetics* Bone Morphogenetic Proteins / metabolism Cell Line DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Gene Expression Regulation* Helix-Loop-Helix Motifs / genetics Mice Mice, Inbred C57BL Nuclear Proteins / genetics Nuclear Proteins / metabolism Osteoblasts Promoter Regions, Genetic* Sequence Deletion Transcription Factors / genetics* Transcription Factors / metabolism Transcription, Genetic* Upstream Stimulatory Factors
IF 2.985
Human and Animal Cells MC3T3-E1(RCB1126)