| RRC ID |
75899
|
| 著者 |
Ebara S, Kawasaki S, Nakamura I, Tsutsumimoto T, Nakayama K, Nikaido T, Takaoka K.
|
| タイトル |
Transcriptional regulation of the mBMP-4 gene through an E-box in the 5'-flanking promoter region involving USF.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
We analyzed the promoter activity of murine bone morphogenetic protein-4 (mBMP-4) and determined that the 5'-flanking region of exon I plays a critical role for transcription and position -265 to -246 of 5'-flanking region of mBMP-4 gene acts as a cis-element important for the regulation of BMP4 transcription in MC3T3E1 cells. By use of site-directed mutagenesis, we have established that the E-box, CACGTG, located within this short region of promoter, is essential for transcriptional activation of the mBMP-4 gene. Upstream stimulatory factor (USF), a member of the helix-loop-helix (HLH) group of regulatory proteins, was found to bind to this E-box using supershift gel mobility analysis. It is proposed that the HLH transcription regulatory proteins play an important role in mBMP-4 gene transcription in this osteoblastic cell line.
|
| 巻・号 |
240(1)
|
| ページ |
136-41
|
| 公開日 |
1997-11-7
|
| DOI |
10.1006/bbrc.1997.7618
|
| PII |
S0006-291X(97)97618-X
|
| PMID |
9367898
|
| MeSH |
Animals
Binding, Competitive / genetics
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins / genetics*
Bone Morphogenetic Proteins / metabolism
Cell Line
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Gene Expression Regulation*
Helix-Loop-Helix Motifs / genetics
Mice
Mice, Inbred C57BL
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Osteoblasts
Promoter Regions, Genetic*
Sequence Deletion
Transcription Factors / genetics*
Transcription Factors / metabolism
Transcription, Genetic*
Upstream Stimulatory Factors
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
