論文 - 詳細
| RRC ID | 75907 |
|---|---|
| 著者 | Abbatemarco S, Bondaz A, Schwager F, Wang J, Hammell CM, Gotta M. |
| タイトル | PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos. |
| ジャーナル | J Cell Sci |
| Abstract |
When exposed to stressful conditions, eukaryotic cells respond by inducing the formation of cytoplasmic ribonucleoprotein complexes called stress granules. Here, we use C. elegans to study two proteins that are important for stress granule assembly in human cells - PQN-59, the human UBAP2L ortholog, and GTBP-1, the human G3BP1 and G3BP2 ortholog. Both proteins assemble into stress granules in the embryo and in the germline when C. elegans is exposed to stressful conditions. Neither of the two proteins is essential for the assembly of stress-induced granules, as shown by the single and combined depletions by RNAi, and neither pqn-59 nor gtbp-1 mutant embryos show higher sensitivity to stress than control embryos. We find that pqn-59 mutants display reduced progeny and a high percentage of embryonic lethality, phenotypes that are not dependent on stress exposure and that are not shared with gtbp-1 mutants. Our data indicate that, in contrast to human cells, PQN-59 and GTBP-1 are not required for stress granule formation but that PQN-59 is important for C. elegans development. |
| 巻・号 | 134(22) |
| 公開日 | 2021-11-15 |
| DOI | 10.1242/jcs.258834 |
| PII | 273437 |
| PMID | 34661238 |
| PMC | PMC8645233 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans Proteins / genetics* Carrier Proteins DNA Helicases Humans Poly-ADP-Ribose Binding Proteins RNA Helicases RNA Recognition Motif Proteins Stress Granules* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1543 |