Reference - Detail
| RRC ID | 75968 |
|---|---|
| Author | Helf MJ, Fox BW, Artyukhin AB, Zhang YK, Schroeder FC. |
| Title | Comparative metabolomics with Metaboseek reveals functions of a conserved fat metabolism pathway in C. elegans. |
| Journal | Nat Commun |
| Abstract |
Untargeted metabolomics via high-resolution mass spectrometry can reveal more than 100,000 molecular features in a single sample, many of which may represent unidentified metabolites, posing significant challenges to data analysis. We here introduce Metaboseek, an open-source analysis platform designed for untargeted comparative metabolomics and demonstrate its utility by uncovering biosynthetic functions of a conserved fat metabolism pathway, α-oxidation, using C. elegans as a model. Metaboseek integrates modules for molecular feature detection, statistics, molecular formula prediction, and fragmentation analysis, which uncovers more than 200 previously uncharacterized α-oxidation-dependent metabolites in an untargeted comparison of wildtype and α-oxidation-defective hacl-1 mutants. The identified metabolites support the predicted enzymatic function of HACL-1 and reveal that α-oxidation participates in metabolism of endogenous β-methyl-branched fatty acids and food-derived cyclopropane lipids. Our results showcase compound discovery and feature annotation at scale via untargeted comparative metabolomics applied to a conserved primary metabolic pathway and suggest a model for the metabolism of cyclopropane lipids. |
| Volume | 13(1) |
| Pages | 782 |
| Published | 2022-2-10 |
| DOI | 10.1038/s41467-022-28391-9 |
| PII | 10.1038/s41467-022-28391-9 |
| PMID | 35145075 |
| PMC | PMC8831614 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Carbon-Carbon Lyases / genetics Carbon-Carbon Lyases / metabolism Fatty Acids / genetics Fatty Acids / metabolism Humans Larva Lipid Metabolism* / genetics Mass Spectrometry Metabolic Networks and Pathways* / genetics Metabolome Metabolomics / methods* Oxidation-Reduction |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 78 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm6725 |