RRC ID 76025
Author Ou HL, Schumacher B.
Title Evaluating DNA damage response through immunofluorescence staining of primordial germ cells in Caenorhabditis elegans L1 larva.
Journal STAR Protoc
Abstract C. elegans L1 larvae have two well-defined primordial germ cells embedded in a niche comprising two somatic gonad precursor cells. Thus, C. elegans provides an ideal model for studying intercellular signaling in response to DNA damage. However, existing staining protocols are focused on worms in later developmental stages and are not optimized for the L1 larvae. Here, we present a revised protocol for assessing the DNA damage response utilizing immunofluorescence staining specifically in C. elegans L1 larva. For complete details on the use and execution of this protocol, please refer to Ou et al. (2019).
Volume 2(2)
Pages 100441
Published 2021-6-18
DOI 10.1016/j.xpro.2021.100441
PII S2666-1667(21)00148-9
PMID 33899022
PMC PMC8056264
MeSH Animals Caenorhabditis elegans / cytology DNA Damage / genetics* DNA, Helminth / analysis DNA, Helminth / chemistry Fluorescent Antibody Technique / methods* Germ Cells / chemistry Germ Cells / cytology* Germ Cells / pathology Larva / cytology Signal Transduction
Resource
C.elegans tm3886 tm684