| RRC ID |
76043
|
| 著者 |
Reilly DK, McGlame EJ, Vandewyer E, Robidoux AN, Muirhead CS, Northcott HT, Joyce W, Alkema MJ, Gegear RJ, Beets I, Srinivasan J.
|
| タイトル |
Distinct neuropeptide-receptor modules regulate a sex-specific behavioral response to a pheromone.
|
| ジャーナル |
Commun Biol
|
| Abstract |
Dioecious species are a hallmark of the animal kingdom, with opposing sexes responding differently to identical sensory cues. Here, we study the response of C. elegans to the small-molecule pheromone, ascr#8, which elicits opposing behavioral valences in each sex. We identify a novel neuropeptide-neuropeptide receptor (NP/NPR) module that is active in males, but not in hermaphrodites. Using a novel paradigm of neuropeptide rescue that we established, we leverage bacterial expression of individual peptides to rescue the sex-specific response to ascr#8. Concurrent biochemical studies confirmed individual FLP-3 peptides differentially activate two divergent receptors, NPR-10 and FRPR-16. Interestingly, the two of the peptides that rescued behavior in our feeding paradigm are related through a conserved threonine, suggesting that a specific NP/NPR combination sets a male state, driving the correct behavioral valence of the ascr#8 response. Receptor expression within pre-motor neurons reveals novel coordination of male-specific and core locomotory circuitries.
|
| 巻・号 |
4(1)
|
| ページ |
1018
|
| 公開日 |
2021-8-31
|
| DOI |
10.1038/s42003-021-02547-7
|
| PII |
10.1038/s42003-021-02547-7
|
| PMID |
34465863
|
| PMC |
PMC8408276
|
| MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins
Carrier Proteins
Hermaphroditic Organisms / genetics
Hermaphroditic Organisms / physiology*
Locomotion* / drug effects
Male
Receptors, Neuropeptide / genetics*
Receptors, Neuropeptide / metabolism
|
| リソース情報 |
| 線虫 |
tm8982
tm1782
tm272 |
