RRC ID 76049
Author Sallee MD, Pickett MA, Feldman JL.
Title Apical PAR complex proteins protect against programmed epithelial assaults to create a continuous and functional intestinal lumen.
Journal Elife
Abstract Sustained polarity and adhesion of epithelial cells is essential for the protection of our organs and bodies, and this epithelial integrity emerges during organ development amidst numerous programmed morphogenetic assaults. Using the developing Caenorhabditis elegans intestine as an in vivo model, we investigated how epithelia maintain their integrity through cell division and elongation to build a functional tube. Live imaging revealed that apical PAR complex proteins PAR-6/Par6 and PKC-3/aPkc remained apical during mitosis while apical microtubules and microtubule-organizing center (MTOC) proteins were transiently removed. Intestine-specific depletion of PAR-6, PKC-3, and the aPkc regulator CDC-42/Cdc42 caused persistent gaps in the apical MTOC as well as in other apical and junctional proteins after cell division and in non-dividing cells that elongated. Upon hatching, gaps coincided with luminal constrictions that blocked food, and larvae arrested and died. Thus, the apical PAR complex maintains apical and junctional continuity to construct a functional intestinal tube.
Volume 10
Published 2021-6-17
DOI 10.7554/eLife.64437
PII 64437
PMID 34137371
PMC PMC8245128
MeSH Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism* Epithelial Cells* / cytology Epithelial Cells* / metabolism Intestinal Mucosa* / cytology Intestinal Mucosa* / metabolism Intestinal Mucosa* / physiology Intestines / cytology Intestines / growth & development Larva / growth & development Microtubule-Organizing Center / metabolism Protein Kinase C / metabolism
C.elegans tm4063