Reference - Detail
| RRC ID | 76079 |
|---|---|
| Author | van der Woude M, Lans H. |
| Title | C. elegans survival assays to discern global and transcription-coupled nucleotide excision repair. |
| Journal | STAR Protoc |
| Abstract |
Global genome nucleotide excision repair (GG-NER) and transcription-coupled nucleotide excision repair (TC-NER) protect cells against a variety of helix-distorting DNA lesions. In C. elegans, GG-NER primarily acts in proliferative germ cells and embryos, while TC-NER acts in post-mitotic somatic cells to maintain transcription. We leverage this difference to distinguish whether proteins function in GG-NER and/or TC-NER by straightforward UV survival assays. Here, we detail a protocol for these assays, using GG-NER factor xpc-1 and TC-NER factor csb-1 as examples. For complete details on the use and execution of this protocol, please refer to Sabatella et al. (2021). |
| Volume | 2(2) |
| Pages | 100586 |
| Published | 2021-6-18 |
| DOI | 10.1016/j.xpro.2021.100586 |
| PII | S2666-1667(21)00293-8 |
| PMID | 34151304 |
| PMC | PMC8192855 |
| MeSH | Animals Caenorhabditis elegans / physiology* DNA Repair* Transcription, Genetic* |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm3886 |